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- Antibody Data
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- References [1]
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- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- NB100-82021 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-82021, RRID:AB_1144538
- Product name
- Rabbit Polyclonal LIM Kinase 1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. LIMK1 (specific to Phospho-Thr508) detects endogenous levels of LIMK1 only when phosphorylated at threonine 508.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.05 ml
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Essential role of GluD1 in dendritic spine development and GluN2B to GluN2A NMDAR subunit switch in the cortex and hippocampus reveals ability of GluN2B inhibition in correcting hyperconnectivity.
Gupta SC, Yadav R, Pavuluri R, Morley BJ, Stairs DJ, Dravid SM
Neuropharmacology 2015 Jun;93:274-84
Neuropharmacology 2015 Jun;93:274-84
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: LIM Kinase 1 [p Thr508] Antibody [NB100-82021] - Analysis of extracts from UV-treated C2C12, PMA-treated Hela and anisomycin-treated 293 cells using LIMK1(Phospho-Thr508) antibody
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: LIM Kinase 1 [p Thr508] Antibody [NB100-82021] - Lane 1 : Hela treated with PMA; Lane 2 : Mouse brain tissue; Lane 3 : Rat brain tissue; Lane 4 : N-peptide blocked Hela treated with PMA; Lane 5 : N-peptide blocked Mouse brain tissue; Lane 6 : N-peptide blocked Rat brain tissue; Lane 7 : P-peptide blocked Hela treated with PMA; Lane 8 : P-peptide blocked Mouse brain tissue; Lane 9 : P-peptide blocked Rat brain tissue; Lysates/proteins at 40 ug per lane. Predicted band size : 72 kDa Observed band size : 72 kDa
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: LIM Kinase 1 [p Thr508] Antibody [NB100-82021] - Immunohistochemical analysis of paraffin- embedded human breast carcinoma tissue, using LIMK1 (phospho- Thr508) antibody
