MAB995-100

antibody from R&D Systems

Targeting: ABCG2 ABCP, BCRP, CD338, EST157481, MXR

Immunocytochemistry (Supportive data in Antibodypedia)

Flow cytometry (Data presented on provider website)

Antibody Data

Product number

MAB995-100

Provider

R&D Systems

Product name

Human ABCG2 Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from hybridoma culture supernatant. Detects human ABCG2 in flow cytometry and immunocytochemistry.

Reactivity

Human

Host

Mouse

Conjugate

Unconjugated

Isotype

IgG

Antibody clone number

5D3

Vial size

100 ug

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

References

Submitted references

Effect of NOS1 regulating ABCG2 expression on proliferation and apoptosis of cervical cancer cells.

Ding M, Zhang H, Liu L, Liang R
Oncology letters 2019 Feb;17(2):1531-1536

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In Vitro Modeling of Blood-Brain Barrier with Human iPSC-Derived Endothelial Cells, Pericytes, Neurons, and Astrocytes via Notch Signaling.

Yamamizu K, Iwasaki M, Takakubo H, Sakamoto T, Ikuno T, Miyoshi M, Kondo T, Nakao Y, Nakagawa M, Inoue H, Yamashita JK
Stem cell reports 2017 Mar 14;8(3):634-647

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The Metastatic Potential and Chemoresistance of Human Pancreatic Cancer Stem Cells.

Bhagwandin VJ, Bishop JM, Wright WE, Shay JW
PloS one 2016;11(2):e0148807

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Mitochondrial localization of ABC transporter ABCG2 and its function in 5-aminolevulinic acid-mediated protoporphyrin IX accumulation.

Kobuchi H, Moriya K, Ogino T, Fujita H, Inoue K, Shuin T, Yasuda T, Utsumi K, Utsumi T
PloS one 2012;7(11):e50082

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CD molecules 2006--human cell differentiation molecules.

Zola H, Swart B, Banham A, Barry S, Beare A, Bensussan A, Boumsell L, D Buckley C, Bühring HJ, Clark G, Engel P, Fox D, Jin BQ, Macardle PJ, Malavasi F, Mason D, Stockinger H, Yang X
Journal of immunological methods 2007 Jan 30;319(1-2):1-5

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Isolation of multipotent neural crest-derived stem cells from the adult mouse cornea.

Yoshida S, Shimmura S, Nagoshi N, Fukuda K, Matsuzaki Y, Okano H, Tsubota K
Stem cells (Dayton, Ohio) 2006 Dec;24(12):2714-22

Immunocytochemistry

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

ABCG2 in JAR Human Cell Line. ATP-Binding Cassette Transporter G2 (ABCG2) was detected in immersion fixed JAR human choriocarcinoma cell line using Mouse Anti-Human ABCG2 Monoclonal Antibody (Catalog # MAB995) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 493-conjugated Anti-Mouse IgG Secondary Antibody (green; Catalog # NL009) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

ABCG2 in Human ABCG2 transfected CHO Chinese Hamster Cell Line. ABCG2 was detected in immersion fixed Human ABCG2 transfected CHO Chinese hamster ovary cell line using Mouse Anti-Human ABCG2 Monoclonal Antibody (Catalog # MAB995) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.