12-8888-42

antibody from Invitrogen Antibodies

Targeting: ABCG2 ABCP, BCRP, CD338, EST157481, MXR

Flow cytometry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

12-8888-42

Provider

Invitrogen Antibodies

Product name

CD338 (ABCG2) Monoclonal Antibody (5D3), PE, eBioscience™

Antibody type

Monoclonal

Antigen

Other

Reactivity

Human

Host

Mouse

Conjugate

Yellow dye

Isotype

IgG

Antibody clone number

5D3

Vial size

100 Tests

Concentration

5 µL/Test

Storage

4° C, store in dark, DO NOT FREEZE!

References

Submitted references

CBP-mediated Wnt3a/β-catenin signaling promotes cervical oncogenesis initiated by Piwil2.

Feng D, Yan K, Liang H, Liang J, Wang W, Yu H, Zhou Y, Zhao W, Dong Z, Ling B
Neoplasia (New York, N.Y.) 2021 Jan;23(1):1-11

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Piwil2 is reactivated by HPV oncoproteins and initiates cell reprogramming via epigenetic regulation during cervical cancer tumorigenesis.

Feng D, Yan K, Zhou Y, Liang H, Liang J, Zhao W, Dong Z, Ling B
Oncotarget 2016 Oct 4;7(40):64575-64588

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Tariquidar Is an Inhibitor and Not a Substrate of Human and Mouse P-glycoprotein.

Weidner LD, Fung KL, Kannan P, Moen JK, Kumar JS, Mulder J, Innis RB, Gottesman MM, Hall MD
Drug metabolism and disposition: the biological fate of chemicals 2016 Feb;44(2):275-82

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Using the BacMam Baculovirus System to Study Expression and Function of Recombinant Efflux Drug Transporters in Polarized Epithelial Cell Monolayers.

Fung KL, Kapoor K, Pixley JN, Talbert DJ, Kwit AD, Ambudkar SV, Gottesman MM
Drug metabolism and disposition: the biological fate of chemicals 2016 Feb;44(2):180-8

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A double blinded, placebo-controlled pilot study to examine reduction of CD34 (+)/CD117 (+)/CD133 (+) lymphoma progenitor cells and duration of remission induced by neoadjuvant valspodar in dogs with large B-cell lymphoma.

Ito D, Childress M, Mason N, Winter A, O'Brien T, Henson M, Borgatti A, Lewellen M, Krick E, Stewart J, Lahrman S, Rajwa B, Scott MC, Seelig D, Koopmeiners J, Ruetz S, Modiano J
F1000Research 2015;4:42

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Canine osteosarcoma cells exhibit resistance to aurora kinase inhibitors.

Cannon CM, Pozniak J, Scott MC, Ito D, Gorden BH, Graef AJ, Modiano JF
Veterinary and comparative oncology 2015 Mar;13(1):48-59

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Lysosomal drug sequestration as a mechanism of drug resistance in vascular sarcoma cells marked by high CSF-1R expression.

Gorden BH, Saha J, Khammanivong A, Schwartz GK, Dickerson EB
Vascular cell 2014;6:20

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ABCG2pos lung mesenchymal stem cells are a novel pericyte subpopulation that contributes to fibrotic remodeling.

Marriott S, Baskir RS, Gaskill C, Menon S, Carrier EJ, Williams J, Talati M, Helm K, Alford CE, Kropski JA, Loyd J, Wheeler L, Johnson J, Austin E, Nozik-Grayck E, Meyrick B, West JD, Klemm DJ, Majka SM
American journal of physiology. Cell physiology 2014 Oct 15;307(8):C684-98

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Human prostate side population cells demonstrate stem cell properties in recombination with urogenital sinus mesenchyme.

Foster BA, Gangavarapu KJ, Mathew G, Azabdaftari G, Morrison CD, Miller A, Huss WJ
PloS one 2013;8(1):e55062

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Transcription factors Sp1 and Sp3 regulate expression of human ABCG2 gene and chemoresistance phenotype.

Yang WJ, Song MJ, Park EY, Lee JJ, Park JH, Park K, Park JH, Kim HP
Molecules and cells 2013 Oct;36(4):368-75

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Targeting the ABCG2-overexpressing multidrug resistant (MDR) cancer cells by PPARγ agonists.

To KK, Tomlinson B
British journal of pharmacology 2013 Nov;170(5):1137-51

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Ethyl 2-amino-6-(3,5-dimethoxyphenyl)-4-(2-ethoxy-2-oxoethyl)-4H-chromene-3-carboxylate (CXL017): a novel scaffold that resensitizes multidrug resistant leukemia cells to chemotherapy.

Das SG, Hermanson DL, Bleeker N, Lowman X, Li Y, Kelekar A, Xing C
ACS chemical biology 2013 Feb 15;8(2):327-35

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Adverse Cell Culture Conditions Mimicking the Tumor Microenvironment Upregulate ABCG2 to Mediate Multidrug Resistance and a More Malignant Phenotype.

Cheng GM, To KK
ISRN oncology 2012;2012:746025

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Regulation and expression of the ATP-binding cassette transporter ABCG2 in human embryonic stem cells.

Padmanabhan R, Chen KG, Gillet JP, Handley M, Mallon BS, Hamilton RS, Park K, Varma S, Mehaffey MG, Robey PG, McKay RD, Gottesman MM
Stem cells (Dayton, Ohio) 2012 Oct;30(10):2175-87

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Surface antigenic profiling of stem cells from human omentum fat in comparison with subcutaneous fat and bone marrow.

Dhanasekaran M, Indumathi S, Kanmani A, Poojitha R, Revathy KM, Rajkumar JS, Sudarsanam D
Cytotechnology 2012 Feb 1;64(5):497-509

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New use for an old drug: inhibiting ABCG2 with sorafenib.

Wei Y, Ma Y, Zhao Q, Ren Z, Li Y, Hou T, Peng H
Molecular cancer therapeutics 2012 Aug;11(8):1693-702

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Identification of proline residues in or near the transmembrane helices of the human breast cancer resistance protein (BCRP/ABCG2) that are important for transport activity and substrate specificity.

Ni Z, Bikadi Z, Shuster DL, Zhao C, Rosenberg MF, Mao Q
Biochemistry 2011 Sep 20;50(37):8057-66

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Structure and function of ABCG2-rich extracellular vesicles mediating multidrug resistance.

Goler-Baron V, Assaraf YG
PloS one 2011 Jan 24;6(1):e16007

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Transmembrane helices 1 and 6 of the human breast cancer resistance protein (BCRP/ABCG2): identification of polar residues important for drug transport.

Ni Z, Bikadi Z, Cai X, Rosenberg MF, Mao Q
American journal of physiology. Cell physiology 2010 Nov;299(5):C1100-9

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Role of basic residues within or near the predicted transmembrane helix 2 of the human breast cancer resistance protein in drug transport.

Cai X, Bikadi Z, Ni Z, Lee EW, Wang H, Rosenberg MF, Mao Q
The Journal of pharmacology and experimental therapeutics 2010 Jun;333(3):670-81

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Structural determinants of imidazoacridinones facilitating antitumor activity are crucial for substrate recognition by ABCG2.

Bram EE, Adar Y, Mesika N, Sabisz M, Skladanowski A, Assaraf YG
Molecular pharmacology 2009 May;75(5):1149-59

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Arginine 383 is a crucial residue in ABCG2 biogenesis.

Polgar O, Ediriwickrema LS, Robey RW, Sharma A, Hegde RS, Li Y, Xia D, Ward Y, Dean M, Ozvegy-Laczka C, Sarkadi B, Bates SE
Biochimica et biophysica acta 2009 Jul;1788(7):1434-43

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Becatecarin (rebeccamycin analog, NSC 655649) is a transport substrate and induces expression of the ATP-binding cassette transporter, ABCG2, in lung carcinoma cells.

Robey RW, Obrzut T, Shukla S, Polgar O, Macalou S, Bahr JC, Di Pietro A, Ambudkar SV, Bates SE
Cancer chemotherapy and pharmacology 2009 Aug;64(3):575-83

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Identification of cancer stem cell-like side population cells in human nasopharyngeal carcinoma cell line.

Wang J, Guo LP, Chen LZ, Zeng YX, Lu SH
Cancer research 2007 Apr 15;67(8):3716-24

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Staining of human CD338-transfected cells with Mouse IgG2b K Isotype Control PE (Product # 12-4732-81) (open histogram) or Anti-Human CD338 (ABCG2) PE (filled histogram). Total viable cells were used for analysis.

Conjugate

Yellow dye

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NULL

Conjugate

Yellow dye

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NULL

Conjugate

Yellow dye

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 7 Piwil2 initiates cell reprogramming by regulating the balance between the acetylation and trimethylation of H3K9 a. Western blot showing significantly induced H3K9 acetylation but reduced H3K9 trimethylation in HaCaT cells transfected with Piwil2 or E6 and E7 compared with those only transfected with vector. b. Co-immunoprecipitation showing that Piwil2, either overexpressed or induced by E6 and E7, interacted with acetylated H3K9. c. EMT markers upregulated in SiHa cells exhibiting Piwil2 overexpression but downregulated in those cells in which Piwil2 was knocked down, as verified by qRT-PCR. d. The proportion of ALDH-, MSCA-1-, and ABCG2-positive cells, determined by FACS in cells with Piwil2 overexpression or knockdown. e. The LD50 dose of cisplatin, detected by CCK8 assay in cells with Piwil2 overexpression or knockdown. The data are presented as the mean+-SD. * P < 0.05 and ** P < 0.01 by Student&apos;s t -test.

Conjugate

Yellow dye

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 4 CBP/beta-catenin promotes the maintenance of stem cell reprogramming by Piwil2. (A) GSEA plot showing significant enrichment of the Wnt/beta-catenin signaling activation modules in HaCaT-Piwil2 cells. (B) Immunoblotting of beta-catenin translocated into the nucleus in HaCaT-Piwil2 cells after treatment with 20 uM ICG-001 or IQ-1 for 24 h. (C) Nuclear lysates from HaCaT-Piwil2 cells treated with 20 uM ICG-001, 20 uM IQ-1, or DMSO were coimmunoprecipitated with antisera to beta-catenin and immunoblotted for CBP and p300. (D and E) The expression of Piwil2 and the ""reprogramming"" factors c-Myc, Nanog, Oct4, Sox2 , and Klf4 was determined by real-time PCR and immunoblotting in HaCaT-Piwil2 cells treated with 20 uM ICG-001, 20 uM IQ-1, or DMSO for 24 h. (F) The proportion of CD49f-, CD338-, OCT4-, and ALDHA1-positive cells, determined by flow cytometry in HaCaT-Piwil2 cells treated with 20 uM ICG-001, 20 uM IQ-1, or DMSO for 24 h. The data are presented as the mean +- SD. * P < 0.05 and ** P < 0.01 by Student's t test. Figure 4

Conjugate

Yellow dye