A700-067-T

antibody from Invitrogen Antibodies

Targeting: MAFA hMafA, RIPE3b1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

A700-067-T

Provider

Invitrogen Antibodies

Product name

MafA Recombinant Rabbit Monoclonal Antibody (BLR067G)

Antibody type

Monoclonal

Antigen

Other

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Antibody clone number

BLR067G

Vial size

10 µL

Concentration

1000 µg/mL

Storage

4° C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human MafA by western blot. Samples: Whole cell lysate (10 µg) from Beta-TC-6, AlphaTC1 Clone 9, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-MafA recombinant monoclonal antibody [BLR067G] (Product # A700-067 lot 3) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG (A120-101P). Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody [BLR057F] (Product # A700-057).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human MafA by western blot. Samples: Whole cell lysate from HEK293T transfected with myc tagged human or mouse MafA, MafB or c-Maf prepared using NETN lysis buffer. Antibody: Rabbit anti-MafA recombinant monoclonal antibody [BLR067G] (Product # A700-067 lot 3) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG (A120-101P). Chemiluminescence with an exposure time of 10 seconds. Lower Panels: Rabbit recombinant monoclonal antibodies to Myc-tag (Product # A191-101), MafB (Product # A700-046), and cMaf (Product # A700-045).

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of mouse MafA in FFPE beta-TC-6 cells by immunocytochemistry. Antibody: Rabbit anti-MafA recombinant monoclonal antibody [BLR067G] (Product # A700-067; lot 3). Secondary: HRP-conjugated goat anti-rabbit IgG (Product # A120-501P). Substrate: DAB.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human MafA by immunohistochemistry. AntibodySample: FFPE section of human pancreas.Antibody: Rabbit anti-MafA recombinant monoclonal antibody (BLR067G) (A700-067 lot 1) used at 1:200.Secondary: HRP-conjugated goat anti-rabbit IgG (A120-501P). Substrate: DAB.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human MafA in FFPE pancreatic tumor by immunohistochemistry. Antibody: Rabbit anti-MafA recombinant monoclonal antibody [BLR067G] (Product # A700-067 lot 3). Secondary: HRP-conjugated goat anti-rabbit IgG (A120-501P). Substrate: DAB.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human MafA (red) by immunohistochemistry. Sample: FFPE section of human pancreatic carcinoma. Antibody: Rabbit anti-MafA recombinant monoclonal antibody [BLR067G] (Product # A700-067 lot 1) used at 1:250. Secondary: HRP-conjugated goat anti-rabbit IgG (A120-501P). Substrate: Opal™. Counterstain: DAPI (blue).