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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunocytochemistry [2]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- MAB0291 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#MAB0291, RRID:AB_1112824
- Product name
- NGFR monoclonal antibody
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against partial recombinant NGFR.
- Isotype
- IgG
- Storage
- Store at 4°C on dry atmosphere.After reconstitution with deionized water, store at -20°C or lower.Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence staining of adult mouse basal forebrain cholinergic neurons with NGFR monoclonal antibody (Cat # MAB0291), with ALEXA488 secondary antibodies.
- Validation comment
- Immunofluorescence
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescent detection of NGFR in cultured mouse NSC-34 cells using a NGFR monoclonal antibody (Cat # MAB0291).
- Validation comment
- Immunocytochemistry
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunohistochemical staining of NGFR in Balb / C mouse brain (septum) using NGFR monoclonal antibody (Cat # MAB0291) . 4 % paraformaldehyde fixed mouse brain free floating sections were incubated with NGFR monoclonal antibody (1 ug / ml) overnight , followed by incubation with biotinylated goat anti -mouse IgG conjugate at a dilution of 1 : 500 and Vector ABC , DAB stained.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Flow cytometry analysis of BSR-p75 fibroblasts transfected with mouse NGFR and endogenously expressed p75NTR analysis on NSC-34 motor neuron cells. X-63 conjugated control IgG represents negative control for all experiments.
- Validation comment
- Flow Cytometry
