AF2446

antibody from Novus Biologicals

Targeting: CHI3L2 YKL-39, YKL39

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

AF2446

Provider

Novus Biologicals

Product name

Goat Polyclonal YM1/Chitinase 3-like 3 Antibody

Antibody type

Polyclonal

Description

Antigen Affinity-purified.

Reactivity

Mouse

Host

Goat

Conjugate

Unconjugated

Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

References

Submitted references

Inflammasome-Independent Role for NLRP3 in Controlling Innate Antihelminth Immunity and Tissue Repair in the Lung.

Chenery AL, Alhallaf R, Agha Z, Ajendra J, Parkinson JE, Cooper MM, Chan BHK, Eichenberger RM, Dent LA, Robertson AAB, Kupz A, Brough D, Loukas A, Sutherland TE, Allen JE, Giacomin PR
Journal of immunology (Baltimore, Md. : 1950) 2019 Nov 15;203(10):2724-2734

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Macrophages promote epithelial proliferation following infectious and non-infectious lung injury through a Trefoil factor 2-dependent mechanism.

Hung LY, Sen D, Oniskey TK, Katzen J, Cohen NA, Vaughan AE, Nieves W, Urisman A, Beers MF, Krummel MF, Herbert DR
Mucosal immunology 2019 Jan;12(1):64-76

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Macrophage polarization and activation at the interface of multi-walled carbon nanotube-induced pulmonary inflammation and fibrosis.

Dong J, Ma Q
Nanotoxicology 2018 Mar;12(2):153-168

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Dual role of YM1+ M2 macrophages in allergic lung inflammation.

Draijer C, Robbe P, Boorsma CE, Hylkema MN, Melgert BN
Scientific reports 2018 Mar 23;8(1):5105

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EMAPII Monoclonal Antibody Ameliorates Influenza A Virus-Induced Lung Injury.

Lu H, Chelvanambi S, Poirier C, Saliba J, March KL, Clauss M, Bogatcheva NV
Molecular therapy : the journal of the American Society of Gene Therapy 2018 Aug 1;26(8):2060-2069

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Diabetic Wounds Exhibit Decreased Ym1 and Arginase Expression with Increased Expression of IL-17 and IL-20.

Finley PJ, DeClue CE, Sell SA, DeBartolo JM, Shornick LP
Advances in wound care 2016 Nov 1;5(11):486-494

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Severe Reduction in Number and Function of Peripheral T Cells Does Not Afford Protection toward Emphysema and Bronchial Remodeling Induced in Mice by Cigarette Smoke.

De Cunto G, Lunghi B, Bartalesi B, Cavarra E, Fineschi S, Ulivieri C, Lungarella G, Lucattelli M
The American journal of pathology 2016 Jul;186(7):1814-1824

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TNFα-stimulated gene-6 (TSG6) activates macrophage phenotype transition to prevent inflammatory lung injury.

Mittal M, Tiruppathi C, Nepal S, Zhao YY, Grzych D, Soni D, Prockop DJ, Malik AB
Proceedings of the National Academy of Sciences of the United States of America 2016 Dec 13;113(50):E8151-E8158

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Smoking p66Shc knocked out mice develop respiratory bronchiolitis with fibrosis but not emphysema.

Lunghi B, De Cunto G, Cavarra E, Fineschi S, Bartalesi B, Lungarella G, Lucattelli M
PloS one 2015;10(3):e0119797

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Biomarkers of disease and treatment in murine and cynomolgus models of chronic asthma.

Louten J, Mattson JD, Malinao MC, Li Y, Emson C, Vega F, Wardle RL, Van Scott MR, Fick RB, McClanahan TK, de Waal Malefyt R, Beaumont M
Biomarker insights 2012;7:87-104

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Lack of IL-4 receptor expression on T helper cells reduces T helper 2 cell polyfunctionality and confers resistance in allergic bronchopulmonary mycosis.

Müller U, Piehler D, Stenzel W, Köhler G, Frey O, Held J, Grahnert A, Richter T, Eschke M, Kamradt T, Brombacher F, Alber G
Mucosal immunology 2012 May;5(3):299-310

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Extrathymically generated regulatory T cells control mucosal TH2 inflammation.

Josefowicz SZ, Niec RE, Kim HY, Treuting P, Chinen T, Zheng Y, Umetsu DT, Rudensky AY
Nature 2012 Feb 8;482(7385):395-9

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Hookworm-induced persistent changes to the immunological environment of the lung.

Reece JJ, Siracusa MC, Southard TL, Brayton CF, Urban JF Jr, Scott AL
Infection and immunity 2008 Aug;76(8):3511-24

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of Mouse YM1/Chitinase 3-like 3 by Simple Western^TM. Simple Western lane view shows lysates of mouse bone marrow, loaded at 0.2 mg/mL. A specific band was detected for YM1/Chitinase 3-like 3 at approximately 49 kDa (as indicated) using 2.5 µg/mL of Goat Anti-Mouse YM1/Chitinase 3-like 3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2446) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of Mouse YM1/Chitinase 3-like 3 by Western Blot. Western blot shows lysates of mouse bone marrow. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Mouse YM1/Chitinase 3-like 3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2446) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for YM1/Chitinase 3-like 3 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.