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LearnPA5-142234
antibody from Invitrogen Antibodies
Targeting: GTF2IRD1
BEN, Cream1, GTF3, MusTRD1, RBAP2, WBSCR11, WBSCR12
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [3]
- Flow cytometry [1]
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- Product number
- PA5-142234 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- GTF2IRD1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody is tested in Peptide ELISA: antibody detection limit dilution 1:64,000.
- Reactivity
- Human
- Host
- Goat
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of GTF2IRD1 in Jurkat nuclear cell lysate (35 µg protein in RIPA buffer) using GTF2IRD1 Polyclonal Antibody (Product # PA5-142234) at 0.5 µg/mL. Detected by chemiluminescence.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of GTF2IRD1 in U2OS cells using GTF2IRD1 Polyclonal Antibody (Product # PA5-142234). Cells were fixed with paraformaldehyde, permeabilized with 0.15% Triton, and incubated with the primary antibody for 1hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL), showing strong nuclear and cytoplasmic staining and some plasma membrane staining . The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of GTF2IRD1 in A431 cells using GTF2IRD1 Polyclonal Antibody (Product # PA5-142234). Cells were fixed with paraformaldehyde, permeabilized with 0.15% Triton, and incubated with the primary antibody for 1hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of GTF2IRD1 in U2OS cells using GTF2IRD1 Polyclonal Antibody (Product # PA5-142234). Cells were fixed with paraformaldehyde, permeabilized with 0.15% Triton, and incubated with the primary antibody for 1hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL), showing strong nuclear and cytoplasmic staining and some plasma membrane staining . The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow Cytometry analysis of GTF2IRD1 in A431 cells (blue line) using GTF2IRD1 Polyclonal Antibody (Product # PA5-142234). Cells were fixed with paraformaldehyde, permeabilized with 0.5% Triton, and incubated with the primary antibody for 1hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (1 µg/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
