PA5-97880

antibody from Invitrogen Antibodies

Targeting: IRX6 IRX-3, IRX7

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-97880

Provider

Invitrogen Antibodies

Product name

IRX6 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Reactivity

Human, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µg

Concentration

3.78 mg/mL

Storage

-20°C or -80°C if preferred

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of IRX6 using a IRX6 Polyclonal antibody (Product # PA5-97880) at a concentration of 3.8 µg/mL. Positive WB detected in: A549 whole cell lysate, Rat heart tissue. A secondary Goat polyclonal antibody to rabbit IgG was applied at a 1:50,000 dilution. Observed band size: 49 kDa.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of IRX6 using a IRX6 Polyclonal antibody (Product # PA5-97880) at a concentration of 3.8 µg/mL. Positive WB detected in: A549 whole cell lysate, Rat heart tissue. A secondary Goat polyclonal antibody to rabbit IgG was applied at a 1:50,000 dilution. Observed band size: 49 kDa.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of IRX6 in paraffin embedded human cervical cancer using a IRX6 polyclonal antibody (Product # PA5-97880) at a dilution of 1:200. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.