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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- ELISA [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- H00006932-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00006932-M01, RRID:AB_548584
- Product name
- Mouse Monoclonal TCF7/TCF1 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. TCF7 - transcription factor 7 (T-cell specific, HMG-box)
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Multiple tumor suppressor microRNAs regulate telomerase and TCF7, an important transcriptional regulator of the Wnt pathway.
Hrdličková R, Nehyba J, Bargmann W, Bose HR Jr
PloS one 2014;9(2):e86990
PloS one 2014;9(2):e86990
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: TCF7/TCF1 Antibody (1D2) [H00006932-M01] - Analysis of TCF7 expression in transfected 293T cell line by TCF7 monoclonal antibody (M01), clone 1D2.Lane 1: TCF7 transfected lysate(41.6 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Sandwich ELISA: TCF7/TCF1 Antibody (1D2) [H00006932-M01] - Detection limit for recombinant GST tagged TCF7 is approximately 0.1ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: TCF7/TCF1 Antibody (1D2) [H00006932-M01] - Analysis of monoclonal antibody to TCF7 on HeLa cell . Antibody concentration 10 ug/ml.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: TCF7/TCF1 Antibody (1D2) [H00006932-M01] - Analysis of monoclonal antibody to TCF7 on formalin-fixed paraffin-embedded human lymph node. Antibody concentration 3 ug/ml.
