- GTX627420 - GeneTex TET1 antibody

Submitted references Mono-ADP-ribosylation of H3R117 traps 5mC hydroxylase TET1 to impair demethylation of tumor suppressor gene TFPI2.

Li M, Tang Y, Li Q, Xiao M, Yang Y, Wang Y
Oncogene 2019 May;38(18):3488-3503

TET1 promotes 5hmC-dependent stemness, and inhibits a 5hmC-independent epithelial-mesenchymal transition, in cervical precancerous lesions.

Su PH, Hsu YW, Huang RL, Chen LY, Chao TK, Liao CC, Chen CW, Wu TI, Mao SP, Balch C, Lai HC
Cancer letters 2019 May 28;450:53-62

DNA Demethylation by DNMT3A and DNMT3B in vitro and of Methylated Episomal DNA in Transiently Transfected Cells.

Chatterjee B, Lin MH, Chen CC, Peng KL, Wu MS, Tseng MC, Chen YJ, Shen CJ
Biochimica et biophysica acta. Gene regulatory mechanisms 2018 Nov;1861(11):1048-1061

TET proteins safeguard bivalent promoters from de novo methylation in human embryonic stem cells.

Verma N, Pan H, Doré LC, Shukla A, Li QV, Pelham-Webb B, Teijeiro V, González F, Krivtsov A, Chang CJ, Papapetrou EP, He C, Elemento O, Huangfu D
Nature genetics 2018 Jan;50(1):83-95

FAM20C could be targeted by TET1 to promote odontoblastic differentiation potential of human dental pulp cells.

Li Q, Yi B, Feng Z, Meng R, Tian C, Xu Q
Cell proliferation 2018 Apr;51(2):e12426

Targeted inhibition of STAT/TET1 axis as a therapeutic strategy for acute myeloid leukemia.

Jiang X, Hu C, Ferchen K, Nie J, Cui X, Chen CH, Cheng L, Zuo Z, Seibel W, He C, Tang Y, Skibbe JR, Wunderlich M, Reinhold WC, Dong L, Shen C, Arnovitz S, Ulrich B, Lu J, Weng H, Su R, Huang H, Wang Y, Li C, Qin X, Mulloy JC, Zheng Y, Diao J, Jin J, Li C, Liu PP, He C, Chen Y, Chen J
Nature communications 2017 Dec 13;8(1):2099

FOXA1 potentiates lineage-specific enhancer activation through modulating TET1 expression and function.

Yang YA, Zhao JC, Fong KW, Kim J, Li S, Song C, Song B, Zheng B, He C, Yu J
Nucleic acids research 2016 Sep 30;44(17):8153-64

MiR-29a promotes cell proliferation and EMT in breast cancer by targeting ten eleven translocation 1.

Pei YF, Lei Y, Liu XQ
Biochimica et biophysica acta 2016 Nov;1862(11):2177-2185

TET1 knockdown inhibits the odontogenic differentiation potential of human dental pulp cells.

Rao LJ, Yi BC, Li QM, Xu Q
International journal of oral science 2016 Jun 30;8(2):110-6

SOX17 is a critical specifier of human primordial germ cell fate.

Irie N, Weinberger L, Tang WW, Kobayashi T, Viukov S, Manor YS, Dietmann S, Hanna JH, Surani MA
Cell 2015 Jan 15;160(1-2):253-68

IGF2BP1 expression in human mesenchymal stem cells significantly affects their proliferation and is under the epigenetic control of TET1/2 demethylases.

Mahaira LG, Katsara O, Pappou E, Iliopoulou EG, Fortis S, Antsaklis A, Fotinopoulos P, Baxevanis CN, Papamichail M, Perez SA
Stem cells and development 2014 Oct 15;23(20):2501-12

Expression features of DNA methylcytosine dioxygenase ten-eleven translocation 1 in human dental pulp cells.

Li Q, Rao L, Zhang D, Xu Q
Journal of endodontics 2014 Nov;40(11):1791-5

A novel cancer-germline transcript carrying pro-metastatic miR-105 and TET-targeting miR-767 induced by DNA hypomethylation in tumors.

Loriot A, Van Tongelen A, Blanco J, Klaessens S, Cannuyer J, van Baren N, Decottignies A, De Smet C
Epigenetics 2014 Aug;9(8):1163-71

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Enhanced validation

Submitted by: GeneTex (provider)
Enhanced method: Genetic validation
Main image
Experimental details: TET1 antibody [GT1462] detects TET1 protein by western blot analysis.A. 50 ?g whole cell lysate/extract from 293T cells transfected with scramble siRNA B. 50 ?g whole cell lysate/extract from TET1-knockdowned 293T cells6% SDS-PAGETET1 antibody [GT1462] (GTX627420) dilution: 1:500 The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
KD Reagent Type: siRNA
Downregulation: >75%
KD Reagent Prod page: http://www.genetex.com
KD Reagent Prod no: GTX627420
KD Reagent Provider: Customized siRNA
Antibody Lot Number: 40878

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Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: TET1 antibody [GT1462] detects TET1 protein by western blot analysis.A. 30 ?g 293T whole cell lysate/extract B. 30 ?g whole cell lysate/extract of FLAG-human TET1-transfected 293T cells5% SDS-PAGETET1 antibody [GT1462] (GTX627420) dilution: 1:500 The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: TET1 antibody detects TET1 protein by Western blot analysis.A. 30 £gg 293T whole cell lysate/extractB. 30 £gg whole cell lysate/extract of DDDDK-human TET1-transfected 293T cells5 % SDS-PAGETET1 antibody (GTX627420) dilution: 1:500

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: NT2D1 whole cell and nuclear extracts (30 ?g) were separated by 5% SDS-PAGE, and the membrane was blotted with TET1 antibody [GT1462] (GTX627420) diluted at 1:500.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: TET1 antibody [GT1462] detects TET1 protein at nucleus by immunofluorescent analysis. Sample: TET1-transfected (right) or untransfected (left) 293T cells were fixed in 4% paraformaldehyde for 15 min. Green: TET1 protein stained by TET1 antibody (GTX627420) diluted at 1:1000. Blue: Hoechst 33342 staining. Scale bar = 10 £gm.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Immunoprecipitation of TET1 protein from NT2D1 whole cell extracts using 5 £gg of TET1 antibody [GT1462] (GTX627420).Western blot analysis was performed using TET1 antibody [GT1462] (GTX627420).EasyBlot anti-Mouse IgG (GTX221667-01) was used as a secondary reagent.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: TET1 antibody [GT1462] detects TET1 protein at nucleus on HeLa xenograft by immunohistochemical analysis. Sample: Paraffin-embedded HeLa xenograft. TET1 antibody [GT1462] (GTX627420) dilution: 1:100.

GTX627420

Antibody data