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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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- Product number
- GTX130453 - Provider product page
- Provider
- GeneTex
- Product name
- PKC alpha antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat, Zebrafish
- Host
- Rabbit
Submitted references 18β-glycyrrhetinic acid inhibits migration and invasion of human gastric cancer cells via the ROS/PKC-α/ERK pathway.
Cai H, Chen X, Zhang J, Wang J
Journal of natural medicines 2018 Jan;72(1):252-259
Journal of natural medicines 2018 Jan;72(1):252-259
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Supportive validation
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- GeneTex (provider)
- Main image
- Experimental details
- Various tissue extracts (50 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PKC alpha antibody (GTX130453) diluted at 1:2000.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Whole cell extract (30 £gg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with PKC alpha antibody (GTX130453) diluted at 1:1000.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- PKC alpha antibody detects PKC alpha protein at cell membrane and cytoplasm by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: PKC alpha protein stained by PKC alpha antibody (GTX130453) diluted at 1:500.Blue: Hoechst 33342 staining.Scale bar = 10 £gm.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- PKC alpha antibody detects PKC alpha protein in the rod bipolar cells by immunohistochemical analysis.Sample: Frozen sectioned adult mouse retina. Green: PKC alpha protein stained by PKC alpha antibody (GTX130453) diluted at 1:250.Blue: Fluoroshield with DAPI (GTX30920).
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- PKC alpha antibody detects PKC alpha protein by immunohistochemical analysis.Sample: Frozen sectioned adult mouse retina. Red: PKC alpha protein stained by PKC alpha antibody (GTX130453) diluted at 1:250.Blue: Fluoroshield with DAPI (GTX30920).
