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antibody from Novus Biologicals
Targeting: PRKCA
PKCA, PKCα
Western blot ELISA Immunocytochemistry Immunoprecipitation
Immunohistochemistry Flow cytometry RadioimmunoassayAntibody data
- Antibody Data
- Antigen structure
- References [24]
- Comments [0]
- Validations
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- NB600-201 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB600-201, RRID:AB_10003372
- Product name
- Mouse Monoclonal PKC alpha Antibody
- Antibody type
- Monoclonal
- Description
- Protein A purified. This antibody is capable of detecting PKC beta as well.
- Reactivity
- Human, Mouse, Rat, Bovine, Canine, Chicken/Avian, Porcine, Rabbit, Sheep, Zebrafish
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Adenosine receptor expression in the adult zebrafish retina.
Network analysis and mechanisms of action of Chinese herb-related natural compounds in lung cancer cells.
A group I metabotropic glutamate receptor controls synaptic gain between rods and rod bipolar cells in the mouse retina.
Olfactory Ensheathing Cells Inhibit Gliosis in Retinal Degeneration by Downregulation of the Müller Cell Notch Signaling Pathway.
Fragile X Mental Retardation Protein expression in the retina is regulated by light.
Novel processes invaginate the pre-synaptic terminal of retinal bipolar cells.
Rod bipolar cells and horizontal cells form displaced synaptic contacts with rods in the outer nuclear layer of the nob2 retina.
Distribution of group-III metabotropic glutamate receptors in the retina.
Ezrin is a downstream effector of trafficking PKC-integrin complexes involved in the control of cell motility.
Specific involvement of PKC-epsilon in sensitization of the neuronal response to painful heat.
Specific involvement of PKC-epsilon in sensitization of the neuronal response to painful heat.
alpha-Tocopherol specifically inactivates cellular protein kinase C alpha by changing its phosphorylation state.
alpha-Tocopherol specifically inactivates cellular protein kinase C alpha by changing its phosphorylation state.
Okadaic acid interferes with phorbol-ester-mediated down-regulation of protein kinase C-alpha, C-delta and C-epsilon.
Okadaic acid interferes with phorbol-ester-mediated down-regulation of protein kinase C-alpha, C-delta and C-epsilon.
The effect of alpha-tocopherol on the synthesis, phosphorylation and activity of protein kinase C in smooth muscle cells after phorbol 12-myristate 13-acetate down-regulation.
The effect of alpha-tocopherol on the synthesis, phosphorylation and activity of protein kinase C in smooth muscle cells after phorbol 12-myristate 13-acetate down-regulation.
Dysregulation of signal transduction pathways as a potential mechanism of nervous system alterations in HIV-1 gp120 transgenic mice and humans with HIV-1 encephalitis.
Unique phosphorylation of protein kinase C-alpha in PC12 cells induces resistance to translocation and down-regulation.
Unique phosphorylation of protein kinase C-alpha in PC12 cells induces resistance to translocation and down-regulation.
Disruption of cellular signaling pathways by daunomycin through destabilization of nonlamellar membrane structures.
Disruption of cellular signaling pathways by daunomycin through destabilization of nonlamellar membrane structures.
Regulation of protein kinase C activity in neuronal differentiation induced by the N-ras oncogene in PC-12 cells.
Regulation of protein kinase C activity in neuronal differentiation induced by the N-ras oncogene in PC-12 cells.
Grillo SL, McDevitt DS, Voas MG, Khan AS, Grillo MA, Stella SL Jr
Purinergic signalling 2019 Sep;15(3):327-342
Purinergic signalling 2019 Sep;15(3):327-342
Network analysis and mechanisms of action of Chinese herb-related natural compounds in lung cancer cells.
Lin YJ, Liang WM, Chen CJ, Tsang H, Chiou JS, Liu X, Cheng CF, Lin TH, Liao CC, Huang SM, Chen J, Tsai FJ, Li TM
Phytomedicine : international journal of phytotherapy and phytopharmacology 2019 May;58:152893
Phytomedicine : international journal of phytotherapy and phytopharmacology 2019 May;58:152893
A group I metabotropic glutamate receptor controls synaptic gain between rods and rod bipolar cells in the mouse retina.
Hellmer CB, Clemons MR, Nawy S, Ichinose T
Physiological reports 2018 Oct;6(20):e13885
Physiological reports 2018 Oct;6(20):e13885
Olfactory Ensheathing Cells Inhibit Gliosis in Retinal Degeneration by Downregulation of the Müller Cell Notch Signaling Pathway.
Xie J, Huo S, Li Y, Dai J, Xu H, Yin ZQ
Cell transplantation 2017 Jun 9;26(6):967-982
Cell transplantation 2017 Jun 9;26(6):967-982
Fragile X Mental Retardation Protein expression in the retina is regulated by light.
Guimarães-Souza EM, Perche O, Morgans CW, Duvoisin RM, Calaza KC
Experimental eye research 2016 May;146:72-82
Experimental eye research 2016 May;146:72-82
Novel processes invaginate the pre-synaptic terminal of retinal bipolar cells.
Zimov S, Yazulla S
Cell and tissue research 2008 Jul;333(1):1-16
Cell and tissue research 2008 Jul;333(1):1-16
Rod bipolar cells and horizontal cells form displaced synaptic contacts with rods in the outer nuclear layer of the nob2 retina.
Bayley PR, Morgans CW
The Journal of comparative neurology 2007 Jan 10;500(2):286-98
The Journal of comparative neurology 2007 Jan 10;500(2):286-98
Distribution of group-III metabotropic glutamate receptors in the retina.
Quraishi S, Gayet J, Morgans CW, Duvoisin RM
The Journal of comparative neurology 2007 Apr 20;501(6):931-43
The Journal of comparative neurology 2007 Apr 20;501(6):931-43
Ezrin is a downstream effector of trafficking PKC-integrin complexes involved in the control of cell motility.
Ng T, Parsons M, Hughes WE, Monypenny J, Zicha D, Gautreau A, Arpin M, Gschmeissner S, Verveer PJ, Bastiaens PI, Parker PJ
The EMBO journal 2001 Jun 1;20(11):2723-41
The EMBO journal 2001 Jun 1;20(11):2723-41
Specific involvement of PKC-epsilon in sensitization of the neuronal response to painful heat.
Cesare P, Dekker LV, Sardini A, Parker PJ, McNaughton PA
Neuron 1999 Jul;23(3):617-24
Neuron 1999 Jul;23(3):617-24
Specific involvement of PKC-epsilon in sensitization of the neuronal response to painful heat.
Cesare P, Dekker LV, Sardini A, Parker PJ, McNaughton PA
Neuron 1999 Jul;23(3):617-24
Neuron 1999 Jul;23(3):617-24
alpha-Tocopherol specifically inactivates cellular protein kinase C alpha by changing its phosphorylation state.
Ricciarelli R, Tasinato A, Clément S, Ozer NK, Boscoboinik D, Azzi A
The Biochemical journal 1998 Aug 15;334 ( Pt 1):243-9
The Biochemical journal 1998 Aug 15;334 ( Pt 1):243-9
alpha-Tocopherol specifically inactivates cellular protein kinase C alpha by changing its phosphorylation state.
Ricciarelli R, Tasinato A, Clément S, Ozer NK, Boscoboinik D, Azzi A
The Biochemical journal 1998 Aug 15;334 ( Pt 1):243-9
The Biochemical journal 1998 Aug 15;334 ( Pt 1):243-9
Okadaic acid interferes with phorbol-ester-mediated down-regulation of protein kinase C-alpha, C-delta and C-epsilon.
Gatti A, Robinson PJ
European journal of biochemistry 1997 Oct 1;249(1):92-7
European journal of biochemistry 1997 Oct 1;249(1):92-7
Okadaic acid interferes with phorbol-ester-mediated down-regulation of protein kinase C-alpha, C-delta and C-epsilon.
Gatti A, Robinson PJ
European journal of biochemistry 1997 Oct 1;249(1):92-7
European journal of biochemistry 1997 Oct 1;249(1):92-7
The effect of alpha-tocopherol on the synthesis, phosphorylation and activity of protein kinase C in smooth muscle cells after phorbol 12-myristate 13-acetate down-regulation.
Clément S, Tasinato A, Boscoboinik D, Azzi A
European journal of biochemistry 1997 Jun 15;246(3):745-9
European journal of biochemistry 1997 Jun 15;246(3):745-9
The effect of alpha-tocopherol on the synthesis, phosphorylation and activity of protein kinase C in smooth muscle cells after phorbol 12-myristate 13-acetate down-regulation.
Clément S, Tasinato A, Boscoboinik D, Azzi A
European journal of biochemistry 1997 Jun 15;246(3):745-9
European journal of biochemistry 1997 Jun 15;246(3):745-9
Dysregulation of signal transduction pathways as a potential mechanism of nervous system alterations in HIV-1 gp120 transgenic mice and humans with HIV-1 encephalitis.
Wyss-Coray T, Masliah E, Toggas SM, Rockenstein EM, Brooker MJ, Lee HS, Mucke L
The Journal of clinical investigation 1996 Feb 1;97(3):789-98
The Journal of clinical investigation 1996 Feb 1;97(3):789-98
Unique phosphorylation of protein kinase C-alpha in PC12 cells induces resistance to translocation and down-regulation.
Gatti A, Robinson PJ
The Journal of biological chemistry 1996 Dec 6;271(49):31718-22
The Journal of biological chemistry 1996 Dec 6;271(49):31718-22
Unique phosphorylation of protein kinase C-alpha in PC12 cells induces resistance to translocation and down-regulation.
Gatti A, Robinson PJ
The Journal of biological chemistry 1996 Dec 6;271(49):31718-22
The Journal of biological chemistry 1996 Dec 6;271(49):31718-22
Disruption of cellular signaling pathways by daunomycin through destabilization of nonlamellar membrane structures.
Escribá PV, Sastre M, García-Sevilla JA
Proceedings of the National Academy of Sciences of the United States of America 1995 Aug 1;92(16):7595-9
Proceedings of the National Academy of Sciences of the United States of America 1995 Aug 1;92(16):7595-9
Disruption of cellular signaling pathways by daunomycin through destabilization of nonlamellar membrane structures.
Escribá PV, Sastre M, García-Sevilla JA
Proceedings of the National Academy of Sciences of the United States of America 1995 Aug 1;92(16):7595-9
Proceedings of the National Academy of Sciences of the United States of America 1995 Aug 1;92(16):7595-9
Regulation of protein kinase C activity in neuronal differentiation induced by the N-ras oncogene in PC-12 cells.
Lacal JC, Cuadrado A, Jones JE, Trotta R, Burstein DE, Thomson T, Pellicer A
Molecular and cellular biology 1990 Jun;10(6):2983-90
Molecular and cellular biology 1990 Jun;10(6):2983-90
Regulation of protein kinase C activity in neuronal differentiation induced by the N-ras oncogene in PC-12 cells.
Lacal JC, Cuadrado A, Jones JE, Trotta R, Burstein DE, Thomson T, Pellicer A
Molecular and cellular biology 1990 Jun;10(6):2983-90
Molecular and cellular biology 1990 Jun;10(6):2983-90
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: PKC alpha Antibody (MC5) [NB600-201] - IHC staining of PKC alpha in mouse brain using DAB with hematoxylin counterstain.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: PKC alpha Antibody (MC5) [NB600-201] - Detection of PKC alpha in Human HeLa Cell Line by Flow Cytometry. Human HeLa cell line was stained with Mouse Anti- PKC alpha Monoclonal Antibody (Catalog # NB600-201, filled histogram), or Mouse IgG2A isotype control (Catalog # MAB003, open histogram) followed by APC-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005).Images may not be copied, printed or otherwise disseminated without express written permission of Novus Biologicals a bio-techne brand.
