HPA042825

antibody from Atlas Antibodies

Targeting: PADI4 PAD, PADI5, PDI4, PDI5

Antibody data

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Validation data

Product number: HPA042825 - Provider product page
Provider: Atlas Antibodies
Proper citation: Atlas Antibodies Cat#HPA042825, RRID:AB_2678189
Product name: Anti-PADI4
Antibody type: Polyclonal
Reactivity: Human
Host: Rabbit
Conjugate: Unconjugated
Antigen sequence: GTLTQLDICSSAPEDCTSFSINASPGVVVDIAHGP PAKKKSTGSSTWPLDPGVEVTLTMKVASGSTGDQK VQISYYGPKTPPVKALLYLTGV
Isotype: IgG
Vial size: 100 µl
Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.

PADI4 protein structure - HPA042825 shown in red.

Enhanced validation

Submitted by: Atlas Antibodies (provider)
Enhanced method: Orthogonal validation
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Experimental details: Immunohistochemistry analysis in human bone marrow and kidney tissues using Anti-PADI4 antibody. Corresponding PADI4 RNA-seq data are presented for the same tissues.
Sample type: HUMAN
Orthogonal method: Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

Enhanced validation

Submitted by: Atlas Antibodies (provider)
Enhanced method: Independent antibody validation
Main image
Experimental details: Immunohistochemical staining of human bone marrow, liver, spleen and testis using Anti-PADI4 antibody HPA042825 (A) shows similar protein distribution across tissues to independent antibody HPA017007 (B).
Antibody #2 product nr: HPA017007
Antibody provider: Atlas Antibodies

Supportive validation

Submitted by: Atlas Antibodies (provider)
Main image
Experimental details: Immunohistochemical staining of human bone marrow shows strong nuclear positivity in hematopoietic cells.

Supportive validation

Submitted by: Atlas Antibodies (provider)
Main image
Experimental details: Immunohistochemical staining of human bone marrow shows high expression.
Sample type: HUMAN

Supportive validation

Submitted by: Atlas Antibodies (provider)
Main image
Experimental details: Immunohistochemical staining of human kidney shows low expression as expected.
Sample type: HUMAN

Supportive validation

Submitted by: Atlas Antibodies (provider)
Main image
Experimental details: Immunohistochemical staining of human spleen using Anti-PADI4 antibody HPA042825.
Sample type: HUMAN

Supportive validation

Submitted by: Atlas Antibodies (provider)
Main image
Experimental details: Immunohistochemical staining of human liver using Anti-PADI4 antibody HPA042825.
Sample type: HUMAN

Supportive validation

Submitted by: Atlas Antibodies (provider)
Main image
Experimental details: Immunohistochemical staining of human testis using Anti-PADI4 antibody HPA042825.
Sample type: HUMAN