17373-1-AP

antibody from Invitrogen Antibodies

Targeting: PADI4 PAD, PADI5, PDI4, PDI5

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

17373-1-AP

Provider

Invitrogen Antibodies

Product name

PADI4 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: EDFLKSVTT LAMKAKCKLT ICPEEENMDD QWMQDEMEIG YIQAPHKTLP VVFDSPRNRG LKEFPIKRVM GPDFGYVTRG PQTGGISGLD SFGNLEVSPP VTVRGKEYPL GRILFGDSCY PSNDSRQMHQ ALQDFLSAQQ VQAPVKLYSD WLSVGHVDEF LSFVPAPDRK GFRLLLASPR SCYKLFQEQQ NEGHGEALLF EGIKKKKQQK IKNILSNKTL REHNSFVERC IDWNRELLKR ELGLAESDII DIPQLFKLKE FSKAEAFFPN MVNMLVLGKH LGIPKPFGPV INGRCCLEEK VCSLLEPLGL QCTFINDFFT YHIRHGEVHC GTNVRRKPFS FKWWNMVP (317-663 aa encoded by BC025718)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.27 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

WB result of PADI4 antibody (17373-1-AP) with purified protein by T.J. Hollingsworth, Ph.D., University of Tennessee Health Sciences Center.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

A549 cells were subjected to SDS PAGE followed by western blot with 17373-1-AP ( PADI4 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

WB result of PADI4 antibody (17373-1-AP) with purified protein by T.J. Hollingsworth, Ph.D., University of Tennessee Health Sciences Center.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of ( 10% Formaldehyde ) fixed A549 cells using 17373-1-AP (PADI4 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 17373-1-AP (PADI4 Antibody) at dilution of 1:200 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 17373-1-AP (PADI4 Antibody) at dilution of 1:200 (under 40x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using 17373-1-AP (PADI4 Antibody) at dilution of 1:200 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using 17373-1-AP (PADI4 Antibody) at dilution of 1:200 (under 40x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human tonsillitis tissue slide using 17373-1-AP ( PADI4 Antibody) at dilution of 1:200 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human tonsillitis tissue slide using 17373-1-AP ( PADI4 Antibody) at dilution of 1:200 (under 40x lens).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-PADI4 (IP:17373-1-AP, 4ug; Detection:17373-1-AP 1:500) with mouse spleen tissue lysate 4800ug.