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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [10]
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- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- V2329 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- Basic Cytokeratin Antibody
- Antibody type
- Monoclonal
- Antigen
- Solubilized cytokeratin extract from human stratum corneum was used as the immunogen.
- Description
- Protein G purified antibody
- Reactivity
- Human, Mouse, Rat, Bovine, Canine, Chicken/Avian, Rabbit, Simian
- Host
- Mouse
- Conjugate
- Unconjugated
- Antibody clone number
- AE3
- Vial size
- 20 µg, 100 µg (with sodium azide), 100 µg (without sodium azide), 7 ml (prediluted for IHC use, if applicable)
- Storage
- Store the Basic Cytokeratin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
Submitted references Immunocytochemical and electrophoretic distribution of cytokeratins in the regenerating epidermis of the lizard Podarcis muralis.
Preferential deimination of keratin K1 and filaggrin during the terminal differentiation of human epidermis.
Dynamics of keratin assembly: exogenous type I keratin rapidly associates with type II keratin in vivo.
Immunohistochemical analysis of keratin distribution in eccrine poroma.
Evidence that the deep keratin filament systems of the Xenopus embryo act to ensure normal gastrulation.
Antigen retrieval in formalin-fixed, paraffin-embedded tissues: an enhancement method for immunohistochemical staining based on microwave oven heating of tissue sections.
Comparison of keratin monoclonal antibodies MAK-6, AE1:AE3, and CAM-5.2.
Monoclonal antibody studies of mammalian epithelial keratins: a review.
Optimal immunoreactivity of keratin proteins in formalin-fixed, paraffin-embedded tissue requires preliminary trypsinization. An immunoperoxidase study of various tumours using polyclonal and monoclonal antibodies.
The use of aIF, AE1, and AE3 monoclonal antibodies for the identification and classification of mammalian epithelial keratins.
Alibardi L, Maurizii MG, Taddei C
Journal of morphology 2000 Dec;246(3):179-91
Journal of morphology 2000 Dec;246(3):179-91
Preferential deimination of keratin K1 and filaggrin during the terminal differentiation of human epidermis.
Senshu T, Kan S, Ogawa H, Manabe M, Asaga H
Biochemical and biophysical research communications 1996 Aug 23;225(3):712-9
Biochemical and biophysical research communications 1996 Aug 23;225(3):712-9
Dynamics of keratin assembly: exogenous type I keratin rapidly associates with type II keratin in vivo.
Miller RK, Khuon S, Goldman RD
The Journal of cell biology 1993 Jul;122(1):123-35
The Journal of cell biology 1993 Jul;122(1):123-35
Immunohistochemical analysis of keratin distribution in eccrine poroma.
Watanabe S, Mogi S, Ichikawa E, Takahashi H, Minami H, Harada S
The American journal of pathology 1993 Jan;142(1):231-9
The American journal of pathology 1993 Jan;142(1):231-9
Evidence that the deep keratin filament systems of the Xenopus embryo act to ensure normal gastrulation.
Klymkowsky MW, Shook DR, Maynell LA
Proceedings of the National Academy of Sciences of the United States of America 1992 Sep 15;89(18):8736-40
Proceedings of the National Academy of Sciences of the United States of America 1992 Sep 15;89(18):8736-40
Antigen retrieval in formalin-fixed, paraffin-embedded tissues: an enhancement method for immunohistochemical staining based on microwave oven heating of tissue sections.
Shi SR, Key ME, Kalra KL
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 1991 Jun;39(6):741-8
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 1991 Jun;39(6):741-8
Comparison of keratin monoclonal antibodies MAK-6, AE1:AE3, and CAM-5.2.
Listrom MB, Dalton LW
American journal of clinical pathology 1987 Sep;88(3):297-301
American journal of clinical pathology 1987 Sep;88(3):297-301
Monoclonal antibody studies of mammalian epithelial keratins: a review.
Sun TT, Tseng SC, Huang AJ, Cooper D, Schermer A, Lynch MH, Weiss R, Eichner R
Annals of the New York Academy of Sciences 1985;455:307-29
Annals of the New York Academy of Sciences 1985;455:307-29
Optimal immunoreactivity of keratin proteins in formalin-fixed, paraffin-embedded tissue requires preliminary trypsinization. An immunoperoxidase study of various tumours using polyclonal and monoclonal antibodies.
Pinkus GS, O'Connor EM, Etheridge CL, Corson JM
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 1985 May;33(5):465-73
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 1985 May;33(5):465-73
The use of aIF, AE1, and AE3 monoclonal antibodies for the identification and classification of mammalian epithelial keratins.
Cooper D, Schermer A, Pruss R, Sun TT
Differentiation; research in biological diversity 1984;28(1):30-5
Differentiation; research in biological diversity 1984;28(1):30-5
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC: FFPE skin stained with Basic Cytokeratin antibody AE3.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- SDS-PAGE Analysis of Purified, BSA-Free Basic Cytokeratin Antibody (clone AE3). Confirmation of Integrity and Purity of the Antibody.
