50-9113-41
antibody from Invitrogen Antibodies
Targeting: CLEC4D
CD368, CLECSF8, Dectin-3, MCL, Mpcl
Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Flow cytometry [1]
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Validation data
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- Product number
- 50-9113-41 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD368 (Clec6) Monoclonal Antibody (9B9), eFluor™ 660, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: This 9B9 monoclonal antibody reacts with human CD368, also known as Clec6 or ClecsF8 or Clec4D. In peripheral blood, this C-type lectin is expressed highly on granulocytes and CD14+CD16- monocytes. Some dendritic cell populations also exhibit low levels of CD368 expression. Studies have demonstrated that this receptor undergoes endocytosis due to its C-type lectin-like domain. CD368 functions as a phagocytic receptor and is involved in proinflammatory cytokine production in cooperation with Toll-like receptors. Finally, CD368 also participates in cellular activation via signaling through Syk kinase. Applications Reported: This 9B9 antibody has been reported for use in flow cytometric analysis. Due to the endocytic nature of Clec6, we recommend fixing your cells prior staining to ensure the maximum possible signal. Applications Tested: This 9B9 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. eFluor® 660 is a replacement for Alexa Fluor® 647. eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome. Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 9B9
- Vial size
- 25 Tests
- Concentration
- 5 µL/Test
- Storage
- 4° C, store in dark, DO NOT FREEZE!
Submitted references The Dectin-2 family of C-type lectin-like receptors: an update.
The C-type lectin receptor CLECSF8 (CLEC4D) is expressed by myeloid cells and triggers cellular activation through Syk kinase.
The human C-type lectin CLECSF8 is a novel monocyte/macrophage endocytic receptor.
Kerscher B, Willment JA, Brown GD
International immunology 2013 May;25(5):271-7
International immunology 2013 May;25(5):271-7
The C-type lectin receptor CLECSF8 (CLEC4D) is expressed by myeloid cells and triggers cellular activation through Syk kinase.
Graham LM, Gupta V, Schafer G, Reid DM, Kimberg M, Dennehy KM, Hornsell WG, Guler R, Campanero-Rhodes MA, Palma AS, Feizi T, Kim SK, Sobieszczuk P, Willment JA, Brown GD
The Journal of biological chemistry 2012 Jul 27;287(31):25964-74
The Journal of biological chemistry 2012 Jul 27;287(31):25964-74
The human C-type lectin CLECSF8 is a novel monocyte/macrophage endocytic receptor.
Arce I, Martínez-Muñoz L, Roda-Navarro P, Fernández-Ruiz E
European journal of immunology 2004 Jan;34(1):210-20
European journal of immunology 2004 Jan;34(1):210-20
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Normal human peripheral blood was incubated with the 1-step Fix/Lyse Solution (10X) (Product # 00-5333-54) and then stained with Anti-Human CD14 PE (Product # 12-0149-42), Anti-Human CD16 FITC (Product # 11-0168-42), and Mouse IgG2b K Isotype Control eFluor® 660 (Product # 50-4732-82) (blue histogram) or Anti-Human CD368 (Clec6) eFluor® 660 (purple histogram). Cells in the granulocyte (left) and monocyte (right) gates were used for analysis.