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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Other assay [1]
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Validation data
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- Product number
- 10358-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- DDX50 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: EGAFSNFPI SEETIKLLKG RGVTYLFPIQ VKTFGPVYEG KDLIAQARTG TGKTFSFAIP LIERLQRNQE TIKKSRSPKV LVLAPTRELA NQVAKDFKDI TRKLSVACFY GGTSYQSQIN HIRNGIDILV GTPGRIKDHL QSGRLDLSKL RHVVLDEVDQ MLDLGFAEQV EDIIHESYKT DSEDNPQTLL FSATCPQWVY KVAKKYMKSR YEQVDLVGKM TQKAATTVEH LAIQCHWSQR PAVIGDV (136-381 aa encoded by BC000272 )
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.27 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HeLa cells were subjected to SDS PAGE followed by western blot with 10358-1-AP (DDX50 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Jurkat cells were subjected to SDS PAGE followed by western blot with 10358-1-AP (DDX50 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of HepG2 cells, using DDX50 antibody 10358-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-DDX50 (IP:10358-1-AP, 4ug; Detection:10358-1-AP 1:800) with HeLa cells lysate 1200ug.
