Antibody data
- Antibody Data
- Antigen structure
- References [14]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- 10528-1-AP - Provider product page
- Provider
- Proteintech Group
- Proper citation
- Proteintech Cat#10528-1-AP, RRID:AB_2092705
- Product name
- DDX21 antibody
- Antibody type
- Polyclonal
- Description
- KD/KO validated DDX21 antibody (Cat. #10528-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IHC, IP, WB,ELISA.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 20ul, 150ul
Submitted references lncRNA SLERT controls phase separation of FC/DFCs to facilitate Pol I transcription.
Nuclear bodies formed by polyQ-ataxin-1 protein are liquid RNA/protein droplets with tunable dynamics.
PRL3-DDX21 Transcriptional Control of Endolysosomal Genes Restricts Melanocyte Stem Cell Differentiation.
Activation of PARP-1 by snoRNAs Controls Ribosome Biogenesis and Cell Growth via the RNA Helicase DDX21.
Dissecting the Role of DDX21 in Regulating Human Cytomegalovirus Replication.
A Survey of DDX21 Activity During Rev/RRE Complex Formation.
SLERT Regulates DDX21 Rings Associated with Pol I Transcription.
DDX21 translocates from nucleus to cytoplasm and stimulates the innate immune response due to dengue virus infection.
Identification of C/EBPβ target genes in ALK+ anaplastic large cell lymphoma (ALCL) by gene expression profiling and chromatin immunoprecipitation.
A quantitative proteomic analysis uncovers the relevance of CUL3 in bladder cancer aggressiveness.
Nucleolar scaffold protein, WDR46, determines the granular compartmental localization of nucleolin and DDX21.
Myb-binding protein 1a (Mybbp1a) regulates levels and processing of pre-ribosomal RNA.
Differential effects of AKT1(p.E17K) expression on human mammary luminal epithelial and myoepithelial cells.
Differential protein expression profiling by iTRAQ-two-dimensional LC-MS/MS of human bladder cancer EJ138 cells transfected with the metastasis suppressor KiSS-1 gene.
Wu M, Xu G, Han C, Luan PF, Xing YH, Nan F, Yang LZ, Huang Y, Yang ZH, Shan L, Yang L, Liu J, Chen LL
Science (New York, N.Y.) 2021 Jul 30;373(6554):547-555
Science (New York, N.Y.) 2021 Jul 30;373(6554):547-555
Nuclear bodies formed by polyQ-ataxin-1 protein are liquid RNA/protein droplets with tunable dynamics.
Zhang S, Hinde E, Parkyn Schneider M, Jans DA, Bogoyevitch MA
Scientific reports 2020 Jan 31;10(1):1557
Scientific reports 2020 Jan 31;10(1):1557
PRL3-DDX21 Transcriptional Control of Endolysosomal Genes Restricts Melanocyte Stem Cell Differentiation.
Johansson JA, Marie KL, Lu Y, Brombin A, Santoriello C, Zeng Z, Zich J, Gautier P, von Kriegsheim A, Brunsdon H, Wheeler AP, Dreger M, Houston DR, Dooley CM, Sims AH, Busch-Nentwich EM, Zon LI, Illingworth RS, Patton EE
Developmental cell 2020 Aug 10;54(3):317-332.e9
Developmental cell 2020 Aug 10;54(3):317-332.e9
Activation of PARP-1 by snoRNAs Controls Ribosome Biogenesis and Cell Growth via the RNA Helicase DDX21.
Kim DS, Camacho CV, Nagari A, Malladi VS, Challa S, Kraus WL
Molecular cell 2019 Sep 19;75(6):1270-1285.e14
Molecular cell 2019 Sep 19;75(6):1270-1285.e14
Dissecting the Role of DDX21 in Regulating Human Cytomegalovirus Replication.
Hao H, Han T, Xuan B, Sun Y, Tang S, Yue N, Qian Z
Journal of virology 2019 Dec 15;93(24)
Journal of virology 2019 Dec 15;93(24)
A Survey of DDX21 Activity During Rev/RRE Complex Formation.
Hammond JA, Zhou L, Lamichhane R, Chu HY, Millar DP, Gerace L, Williamson JR
Journal of molecular biology 2018 Feb 16;430(4):537-553
Journal of molecular biology 2018 Feb 16;430(4):537-553
SLERT Regulates DDX21 Rings Associated with Pol I Transcription.
Xing YH, Yao RW, Zhang Y, Guo CJ, Jiang S, Xu G, Dong R, Yang L, Chen LL
Cell 2017 May 4;169(4):664-678.e16
Cell 2017 May 4;169(4):664-678.e16
DDX21 translocates from nucleus to cytoplasm and stimulates the innate immune response due to dengue virus infection.
Dong Y, Ye W, Yang J, Han P, Wang Y, Ye C, Weng D, Zhang F, Xu Z, Lei Y
Biochemical and biophysical research communications 2016 Apr 29;473(2):648-53
Biochemical and biophysical research communications 2016 Apr 29;473(2):648-53
Identification of C/EBPβ target genes in ALK+ anaplastic large cell lymphoma (ALCL) by gene expression profiling and chromatin immunoprecipitation.
Bonzheim I, Irmler M, Klier-Richter M, Steinhilber J, Anastasov N, Schäfer S, Adam P, Beckers J, Raffeld M, Fend F, Quintanilla-Martinez L
PloS one 2013;8(5):e64544
PloS one 2013;8(5):e64544
A quantitative proteomic analysis uncovers the relevance of CUL3 in bladder cancer aggressiveness.
Grau L, Luque-Garcia JL, González-Peramato P, Theodorescu D, Palou J, Fernandez-Gomez JM, Sánchez-Carbayo M
PloS one 2013;8(1):e53328
PloS one 2013;8(1):e53328
Nucleolar scaffold protein, WDR46, determines the granular compartmental localization of nucleolin and DDX21.
Hirai Y, Louvet E, Oda T, Kumeta M, Watanabe Y, Horigome T, Takeyasu K
Genes to cells : devoted to molecular & cellular mechanisms 2013 Sep;18(9):780-97
Genes to cells : devoted to molecular & cellular mechanisms 2013 Sep;18(9):780-97
Myb-binding protein 1a (Mybbp1a) regulates levels and processing of pre-ribosomal RNA.
Hochstatter J, Hölzel M, Rohrmoser M, Schermelleh L, Leonhardt H, Keough R, Gonda TJ, Imhof A, Eick D, Längst G, Németh A
The Journal of biological chemistry 2012 Jul 13;287(29):24365-77
The Journal of biological chemistry 2012 Jul 13;287(29):24365-77
Differential effects of AKT1(p.E17K) expression on human mammary luminal epithelial and myoepithelial cells.
Salhia B, Van Cott C, Tegeler T, Polpitiya A, Duquette RA, Gale M, Hostteter G, Petritis K, Carpten J
Human mutation 2012 Aug;33(8):1216-27
Human mutation 2012 Aug;33(8):1216-27
Differential protein expression profiling by iTRAQ-two-dimensional LC-MS/MS of human bladder cancer EJ138 cells transfected with the metastasis suppressor KiSS-1 gene.
Ruppen I, Grau L, Orenes-Piñero E, Ashman K, Gil M, Algaba F, Bellmunt J, Sánchez-Carbayo M
Molecular & cellular proteomics : MCP 2010 Oct;9(10):2276-91
Molecular & cellular proteomics : MCP 2010 Oct;9(10):2276-91
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Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- HepG2 cells were subjected to SDS PAGE followed by western blot with 10528-1-AP(DDX21 antibody) at dilution of 1:2000
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of HepG2 cells, using DDX21 antibody 10528-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunohistochemical of paraffin-embedded human colon cancer using 10528-1-AP(DDX21 antibody) at dilution of 1:100 (under 10x lens)
- Sample type
- tissue