PAB23032

antibody from Abnova Corporation

Targeting: DDX21 GURDB, RH-II/GU

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB23032

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB23032, RRID:AB_11118716

Product name

DDX21 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant DDX21.

Antigen sequence

KPKSDKTEEIAEEEETVFPKAKQVKKKAEPSEVDM
NSPKSKKAKKKEEPSQNDISPKTKSLRKKKEPIEK
KVV


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: RT-4, Lane 2: U-251 MG, Lane 3: Human Plasma, Lane 4: Liver, Lane 5: Tonsil with DDX21 polyclonal antibody (Cat # PAB23032) at 1:250-1:500 dilution.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line U-251 MG with DDX21 polyclonal antibody (Cat # PAB23032) at 1-4 ug/mL dilution shows positivity in nucleus and nucleoli.

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human stomach with DDX21 polyclonal antibody (Cat # PAB23032) shows strong nucleolar positivity in glandular cells at 1:200-1:500 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)