NBP1-83310

antibody from Novus Biologicals

Targeting: DDX21 GURDB, RH-II/GU

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Chromatin Immunoprecipitation (Data presented on provider website)

Antibody Data

Product number

NBP1-83310

Provider

Novus Biologicals

Proper citation

Novus Cat#NBP1-83310, RRID:AB_11027665

Product name

Rabbit Polyclonal DDX21 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Specificity of human DDX21 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.

Reactivity

Human, Mouse, Zebrafish

Host

Rabbit

Isotype

IgG

Vial size

0.1 ml

Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

References

Submitted references

RNA helicase DDX21 mediates nucleotide stress responses in neural crest and melanoma cells.

Santoriello C, Sporrij A, Yang S, Flynn RA, Henriques T, Dorjsuren B, Custo Greig E, McCall W, Stanhope ME, Fazio M, Superdock M, Lichtig A, Adatto I, Abraham BJ, Kalocsay M, Jurynec M, Zhou Y, Adelman K, Calo E, Zon LI
Nature cell biology 2020 Apr;22(4):372-379

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Enhancer-mediated enrichment of interacting JMJD3-DDX21 to ENPP2 locus prevents R-loop formation and promotes transcription.

Argaud D, Boulanger MC, Chignon A, Mkannez G, Mathieu P
Nucleic acids research 2019 Sep 19;47(16):8424-8438

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RNA Targets Ribogenesis Factor WDR43 to Chromatin for Transcription and Pluripotency Control.

Bi X, Xu Y, Li T, Li X, Li W, Shao W, Wang K, Zhan G, Wu Z, Liu W, Lu JY, Wang L, Zhao J, Wu J, Na J, Li G, Li P, Shen X
Molecular cell 2019 Jul 11;75(1):102-116.e9

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Tissue-selective effects of nucleolar stress and rDNA damage in developmental disorders.

Calo E, Gu B, Bowen ME, Aryan F, Zalc A, Liang J, Flynn RA, Swigut T, Chang HY, Attardi LD, Wysocka J
Nature 2018 Feb 1;554(7690):112-117

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RNA helicase DDX21 coordinates transcription and ribosomal RNA processing.

Calo E, Flynn RA, Martin L, Spitale RC, Chang HY, Wysocka J
Nature 2015 Feb 12;518(7538):249-53

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Nuclear myosin 1 is in complex with mature rRNA transcripts and associates with the nuclear pore basket.

Obrdlik A, Louvet E, Kukalev A, Naschekin D, Kiseleva E, Fahrenkrog B, Percipalle P
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2010 Jan;24(1):146-57

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: DDX21 Antibody [NBP1-83310] - Analysis in human cell lines Caco-2 and HeLa using Anti-DDX21 antibody. Corresponding DDX21 RNA-seq data are presented for the same cell lines. Loading control: Anti-GAPDH.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: DDX21 Antibody [NBP1-83310] - Analysis using Anti-DDX21 antibody NBP1-83310 (A) shows similar pattern to independent antibody NBP2-38311 (B).

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: DDX21 Antibody [NBP1-83310] - Staining of human appendix shows high expression.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: DDX21 Antibody [NBP1-83310] - Staining of human skeletal muscle shows low expression as expected.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: DDX21 Antibody [NBP1-83310] - Staining in human appendix and skeletal muscle tissues using anti-DDX21 antibody. Corresponding DDX21 RNA-seq data are presented for the same tissues.