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LearnNB100-1718
antibody from Novus Biologicals
Targeting: DDX21
GURDB, RH-II/GU
Western blot
Immunocytochemistry Immunoprecipitation Immunohistochemistry Flow cytometry Chromatin ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [3]
- Flow cytometry [1]
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- Product number
- NB100-1718 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-1718, RRID:AB_2092708
- Product name
- Rabbit Polyclonal DDX21 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse, Zebrafish
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references DNA-PKcs has KU-dependent function in rRNA processing and haematopoiesis.
RNA helicase DDX21 mediates nucleotide stress responses in neural crest and melanoma cells.
SIRT7 and the DEAD-box helicase DDX21 cooperate to resolve genomic R loops and safeguard genome stability.
Shao Z, Flynn RA, Crowe JL, Zhu Y, Liang J, Jiang W, Aryan F, Aoude P, Bertozzi CR, Estes VM, Lee BJ, Bhagat G, Zha S, Calo E
Nature 2020 Mar;579(7798):291-296
Nature 2020 Mar;579(7798):291-296
RNA helicase DDX21 mediates nucleotide stress responses in neural crest and melanoma cells.
Santoriello C, Sporrij A, Yang S, Flynn RA, Henriques T, Dorjsuren B, Custo Greig E, McCall W, Stanhope ME, Fazio M, Superdock M, Lichtig A, Adatto I, Abraham BJ, Kalocsay M, Jurynec M, Zhou Y, Adelman K, Calo E, Zon LI
Nature cell biology 2020 Apr;22(4):372-379
Nature cell biology 2020 Apr;22(4):372-379
SIRT7 and the DEAD-box helicase DDX21 cooperate to resolve genomic R loops and safeguard genome stability.
Song C, Hotz-Wagenblatt A, Voit R, Grummt I
Genes & development 2017 Jul 1;31(13):1370-1381
Genes & development 2017 Jul 1;31(13):1370-1381
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: DDX21 Antibody [NB100-1718] - Detection of Human DDX21 on HeLa whole cell lystate using NB100-1718. DDX21 was also immunoprecipitated using rabbit anti-DDX21 antibodies NB100-1716 and NB100-1717.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: DDX21 Antibody [NB100-1718] - FFPE section of human larynx squamous cell carcinoma. Antibody used at a dilution of 1:250. Detection: DAB staining using Immunohistochemistry Accessory Kit.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: DDX21 Antibody [NB100-1718] - FFPE section of mouse squamous cell carcinoma. Antibody used at a dilution of 1:250. Detection: DAB staining using Immunohistochemistry Accessory Kit.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: DDX21 Antibody [NB100-1718] - Sample: FFPE section of human breast carcinoma (left) and mouse squamous cell carcinoma (right). Antibody: Affinity purified rabbit anti-DDX21 used at a dilution of 1:200 (1ug/ml). Detection: DAB.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: DDX21 Antibody [NB100-1718] - An intracellular stain was performed on Jurkat Cells with DDX21Antibody NB100-1718 and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG APC-conjugated Secondary Antibody (R&D Systems, F0111).
