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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [6]
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- Validations
- Western blot [3]
- Flow cytometry [1]
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- Product number
- PA1-734 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SCAMP2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- PA1-734 detects secretory carrier membrane protein 2 (SCAMP 2) from mouse testes and AtT20 cell extract as well as Chinese hamster ovary (CHO) cell extract.
- Concentration
- 1 mg/mL
Submitted references Tissue expression of the vesicle protein pantophysin.
Tissue expression of the vesicle protein pantophysin.
Tyrosine phosphorylation of selected secretory carrier membrane proteins, SCAMP1 and SCAMP3, and association with the EGF receptor.
Tyrosine phosphorylation of selected secretory carrier membrane proteins, SCAMP1 and SCAMP3, and association with the EGF receptor.
Three mammalian SCAMPs (secretory carrier membrane proteins) are highly related products of distinct genes having similar subcellular distributions.
Three mammalian SCAMPs (secretory carrier membrane proteins) are highly related products of distinct genes having similar subcellular distributions.
Windoffer R, Borchert-Stuhlträger M, Haass NK, Thomas S, Hergt M, Bulitta CJ, Leube RE
Cell and tissue research 1999 Jun;296(3):499-510
Cell and tissue research 1999 Jun;296(3):499-510
Tissue expression of the vesicle protein pantophysin.
Windoffer R, Borchert-Stuhlträger M, Haass NK, Thomas S, Hergt M, Bulitta CJ, Leube RE
Cell and tissue research 1999 Jun;296(3):499-510
Cell and tissue research 1999 Jun;296(3):499-510
Tyrosine phosphorylation of selected secretory carrier membrane proteins, SCAMP1 and SCAMP3, and association with the EGF receptor.
Wu TT, Castle JD
Molecular biology of the cell 1998 Jul;9(7):1661-74
Molecular biology of the cell 1998 Jul;9(7):1661-74
Tyrosine phosphorylation of selected secretory carrier membrane proteins, SCAMP1 and SCAMP3, and association with the EGF receptor.
Wu TT, Castle JD
Molecular biology of the cell 1998 Jul;9(7):1661-74
Molecular biology of the cell 1998 Jul;9(7):1661-74
Three mammalian SCAMPs (secretory carrier membrane proteins) are highly related products of distinct genes having similar subcellular distributions.
Singleton DR, Wu TT, Castle JD
Journal of cell science 1997 Sep;110 ( Pt 17):2099-107
Journal of cell science 1997 Sep;110 ( Pt 17):2099-107
Three mammalian SCAMPs (secretory carrier membrane proteins) are highly related products of distinct genes having similar subcellular distributions.
Singleton DR, Wu TT, Castle JD
Journal of cell science 1997 Sep;110 ( Pt 17):2099-107
Journal of cell science 1997 Sep;110 ( Pt 17):2099-107
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot of SCAMP 2 on AtT20 extract using Product # PA1-734.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on membrane extracts (30 µglysate) of U-87 MG (Lane 1), Neuro-2a (Lane 2), SH-SY5Y (Lane 3), SK-N-AS (Lane 4), and T98G (Lane 5). The blots were probed with Rabbit Anti-SCAMP2 Polyclonal Antibody (Product # PA1-734, 2 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). A 36 kDa band corresponding to SCAMP1 was observed across the cell lines and tissues tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0321BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5 % skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Knockdown of SCAMP2 was achieved by transfecting MCF-7 cells with SCAMP2 specific siRNAs (Silencer® select Product # s19575, s19574). Western blot analysis (Fig a) was performed using whole cell extracts from the SCAMP2 knock down cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blots were probed with Anti- SCAMP2 Rabbit polyclonal Antibody (Product # PA1-734, 1µg/mL) and Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to SCAMP2.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry analysis of SCAMP2 was done on F9 cells. Cells were fixed with 70% ethanol for 10 minutes, permeabilized with 0.25% Triton™ X-100 for 20 minutes, and blocked with 5% BSA for 30 minutes at room temperature. Cells were labeled with SCAMP2 Rabbit Polyclonal Antibody (PA1734, red histogram) or with rabbit isotype control (pink histogram) at 3-5 ug/million cells in 2.5% BSA. After incubation at room temperature for 2 hours, the cells were labeled with Alexa Fluor® 488 Goat Anti-Rabbit Secondary Antibody (A11008) at a dilution of 1:400 for 30 minutes at room temperature. The representative 10,000 cells were acquired and analyzed for each sample using an Attune® Acoustic Focusing Cytometer. The purple histogram represents unstained control cells and the green histogram represents no-primary-antibody control..
