PA1-32129

antibody from Invitrogen Antibodies

Targeting: ABCA1 ABC1, HDLDT1, TGD

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Gel shift (Recommended by provider)

Chromatin Immunoprecipitation (Recommended by provider)

Antibody Data

Product number

PA1-32129

Provider

Invitrogen Antibodies

Product name

ABCA1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Reactivity

Human, Mouse, Rat, Canine, Chicken/Avian, Hamster, Porcine

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of ABCA1 in mouse peritoneal macrophages: T09 uninduced lysate (lane 1) and T09 induced lysate (lane 2) using an ABCA1 polyclonal antibody (Product # PA1-32129).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of RAW264.7 cells treated with vehicle (-) or 9-cisretinoic acid and 22rhydroxycholesterol (+) using ABCA1 Polyclonal Antibody (Product # PA1-32129). Loading: 40 µg.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of mouse peritoneal macrophages using ABCA1 Polyclonal Antibody (Product # PA1-32129). 1: T09 cell lysate. 2: Induced T09 cell lysate.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of post-nuclear lysate of raw macrophages treated with 9-cisretinoic acid and 22R-hydroxycholesterol using ABCA1 Polyclonal Antibody (Product # PA1-32129). Loading: 40 µg.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot was performed using anti-ABCA1 Polyclonal Antibody (Product # PA1-32129) and a 254 kDa band corresponding to ABCA1 was increased upon THP-1 differentiated to macrophages and LPS treatment in THP-1. Also, the expression was observed to be high in Mouse and Rat Liver when compared to other tissues. Few uncharacterized bands were also observed across the samples tested. Membrane enriched extracts (30 µg lysate) of THP-1 (Lane 1), THP-1 differentiated to macrophages (Lane 2), THP-1 treated with LPS (1 µg/mL for 24hrs) (Lane 3), Mouse Liver (Lane 4), Rat Liver (Lane 5), Mouse Brown Adipose (Lane 6) and Mouse Heart (Lane 7) were electrophoresed using NuPAGE™ 3-8% Tris-Acetate Protein Gels (Product # EA0378BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using SuperSignal™ West Dura Extended Duration Substrate (Product # 34076).

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of ABCA1 in HepG2 cells. Cells were fixed with 10% formalin, permeabilized with 0.5% Triton-X100 and ABCA1 Polyclonal Antibody (Product # PA1-32129) (Green) at a dilution of 5 µg/mL was used as a primary antibody. Red : Tubulin. Blue: DAPI.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of ABCA1 in HepG2 cells(serum starvation 24hrs and treated with 1uM TO9). Cells were fixed with 10% formalin, permeabilized with 0.5% Triton-X100 and ABCA1 Polyclonal Antibody (Product # PA1-32129) (Green) at a dilution of 5 µg/mL was used as a primary antibody. Red : Tubulin. Blue: DAPI.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry (Paraffin) analysis of ABCA1 in prostate tissue using ABCA1 Polyclonal Antibody (Product # PA1-32129).

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow Cytometry analysis of Glypican 3 was performed in HeLa cells using Glypican 3 Monoclonal Antibody (GT764) (Product # MA5-31547) (Purple) at a dilution of 1:400. M1 is defined by unstained cells.