209-401-B96

antibody from Rockland Immunochemicals, Inc.

Targeting: IL9 HP40, IL-9, P40

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

209-401-B96

Provider

Rockland Immunochemicals, Inc.

Proper citation

Rockland Cat#209-401-B96, RRID:AB_10705048

Product name

Anti-Human IL-9 (RABBIT) Antibody - 209-401-B96

Antibody type

Polyclonal

Vial size

100 µl

Western blot

Supportive validation

Submitted by

Rockland Immunochemicals, Inc. (provider)

Main image

Experimental details

Rockland's anti-Human IL-9 antibody shows detection of a band ~15 kDa in size corresponding to recombinant human IL-9. The identity of the faint higher molecular weight band may represent a homodimer. Molecular weight markers are also shown (left). After transfer, the membrane was blocked overnight with 3% BSA in TBS followed by reaction with primary antibody at a 1:1,000 dilution. Detection occurred using peroxidase conjugated anti-Rabbit IgG (p/n 611-103-122) secondary antibody diluted 1:40,000 in blocking buffer (p/n MB-070) for 30 min at RT followed by reaction with FemtoMax™ chemiluminescent substrate. Image was captured using VersaDoc™ MP 4000 imaging system (Bio-Rad).

Validation comment

Western Blot