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LearnPA5-77319
antibody from Invitrogen Antibodies
Targeting: TRPV4
CMT2C, OTRPC4, TRP12, VR-OAC, VRL-2, VROAC
Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- PA5-77319 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TRPV4 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 50 µL
- Concentration
- 0.8 mg/mL
- Storage
- -20°C
Submitted references TRPV4 antagonists ameliorate ventriculomegaly in a rat model of hydrocephalus.
Hochstetler AE, Smith HM, Preston DC, Reed MM, Territo PR, Shim JW, Fulkerson D, Blazer-Yost BL
JCI insight 2020 Sep 17;5(18)
JCI insight 2020 Sep 17;5(18)
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ND7/23 cell line lysate with TRPV4 polyclonal antibody (Product # PA5-77319) using a dilution of 1:200.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ND7/23 cell line lysate with TRPV4 polyclonal antibody (Product # PA5-77319) using a dilution of 1:200.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of TRPV4 in paraformaldehyde-fixed and permeabilized rat dorsal root ganglion. A, D) Samples were probed with TRPV4 polyclonal antibody (Product # PA5-77319) at a dilution of 1:500, and incubated with goat-anti-rabbit-AlexaFluor-555 and Hoechst. B, E) Shows nuclei stained image. C) Shows merged images of Panels A and B. F) Shows merged images of Panels D and E.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of TRPV4 in frozen ratdorsal root ganglion. A) Samples were probed with TRPV4 polyclonal antibody (Product # PA5-77319) and incubated with parvalbumin (green). B) Dorsal root ganglion stained image. C) Confocal merged image.
