11400-1-AP

antibody from Invitrogen Antibodies

Targeting: ASRGL1 ALP, ALP1, FLJ22316

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

11400-1-AP

Provider

Invitrogen Antibodies

Product name

ASRGL1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: MNPIVVVHG GGAGPISKDR KERVHQGMVR AATVGYGILR EGGSAVDAVE GAVVALEDDP EFNAGCGSVL NTNGEVEMDA SIMDGKDLSA GAVSAVQCIA NPIKLARLVM EKTPHCFLTD QGAAQFAAAM GVPEIPGEKL VTERNKKRLE KEKHEKGAQK TDCQKNLGTV GAVALDCKGN VAYATSTGGI VNKMVGRVGD SPCLGAGGYA DNDIGAVSTT GHGESILKVN LARLTLFHIE QGKTVEEAAD LSLGYMKSRV KGLGGLIVVS KTGDWVAKWT STSMPWAAAK DGKLHFGIDP DDTTITDLP (1-308 aa encoded by BC021295 )

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.37 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HeLa cells were subjected to SDS PAGE followed by western blot with 11400-1-AP (ASRGL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Mouse testis tissue were subjected to SDS PAGE followed by western blot with 11400-1-AP (ASRGL1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of ( 10% Formaldehyde ) fixed HeLa cells using 11400-1-AP (ASRGL1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human gliomas using 11400-1-AP (ASRGL1 antibody) at dilution of 1:50 (under 10x lens).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-ASRGL1 (IP:11400-1-AP, 4ug; Detection:11400-1-AP 1:500) with HeLa cells lysate 3200ug.