Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Immunohistochemistry [1]
- Blocking/Neutralizing [1]
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Validation data
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- Product number
- AF4218 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Sheep Polyclonal Uteroglobin/SCGB1A1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human Uteroglobin/SCGB1A1 in direct ELISAs.
- Reactivity
- Human
- Host
- Sheep
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Oligodendroglia Are Particularly Vulnerable to Oxidative Damage after Neurotrauma In Vivo.
Giacci MK, Bartlett CA, Smith NM, Iyer KS, Toomey LM, Jiang H, Guagliardo P, Kilburn MR, Fitzgerald M
The Journal of neuroscience : the official journal of the Society for Neuroscience 2018 Jul 18;38(29):6491-6504
The Journal of neuroscience : the official journal of the Society for Neuroscience 2018 Jul 18;38(29):6491-6504
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Uteroglobin/SCGB1A1 in Human Endometrial Cancer Tissue. Uteroglobin/SCGB1A1 was detected in immersion fixed paraffin-embedded sections of human endometrial cancer tissue using Human Uteroglobin/SCGB1A1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4218) at 3 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm of epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Cell Adhesion Mediated by Uteroglobin and Neutralization by Human Uteroglobin Antibody. Recombinant Human Uteroglobin (Catalog # 4218-UT), immobilized onto a microplate, supports the adhesion of A549 human lung carcinoma cell line in a dose-dependent manner (orange line). Adhesion elicited by Recombinant Human Uteroglobin (5 µg/mL) is neutralized (green line) by increasing concentrations of Human Uteroglobin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4218). The ND50 is typically 0.3-1.2 µg/mL.