LS-C817559

UCKL1 antibody from LifeSpan BioSciences, Inc.

FLJ20517, URKL1

Western blot

Supportive data in Antibodypedia

Immunocytochemistry

Data presented on provider website

Immunoprecipitation

Supportive data in Antibodypedia

Immunohistochemistry

Data presented on provider website

Provider product page for LS-C817559

Antibody Data

Product number

LS-C817559

Provider

LifeSpan BioSciences, Inc.

Product name

UCKL1 Antibody LS-C817559

Provider product page

Antibody type

Polyclonal

Description

Protein A affinity chromatography

Reactivity

Human

Host

Rabbit

Isotype

IgG

Storage

Store at 2°C to 8°C for up to 1 month. Aliquot and store at -20°C to -80°C for up to 1 year. Avoid freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

LifeSpan BioSciences, Inc. (provider)

Main image

Experimental details

Anti-UCKL1 rabbit polyclonal antibody at 1:500 dilution. Lane A: 293T Whole Cell Lysate. Lane B: HeLa Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 61 kDa. Observed band size: 61 kDa. (We are unsure as to the identity of these extra bands.)

Immunoprecipitation

Supportive validation

Submitted by

LifeSpan BioSciences, Inc. (provider)

Main image

Experimental details

UCKL1 was immunoprecipitated using: Lane A: 0.5 mg HeLa Whole Cell Lysate. 4 uL anti-UCKL1 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-UCKL1 rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 61 kDa. Observed band size: 61 kDa.