PA5-118336

UCKL1 antibody from Invitrogen Antibodies

FLJ20517, URKL1

Western blot

Supportive data in Antibodypedia

Immunocytochemistry

Supportive data in Antibodypedia

Immunoprecipitation

Data presented on provider website

Immunohistochemistry

Supportive data in Antibodypedia

Other assay

Supportive data in Antibodypedia

Provider product page for PA5-118336

Antibody Data

Product number

PA5-118336

Provider

Invitrogen Antibodies

Product name

UCKL1 Polyclonal Antibody

Provider product page

Antibody type

Polyclonal

Antigen

Other

Description

Applications Tested: This UCHT2 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot of UCKL1 in Lane A: 293T Whole Cell Lysate, Lane B: HeLa Whole Cell Lysate. Samples (30 µg per lane) were incubated with polyclonal antibody (Product # PA5-118336) with a dilution of 1:500 , followed by Goat Anti-Rabbit IgG (H+L)/HRP using a dilution of 1:10,000. Assay was performed under reducing conditions. Predicted band size: 61 kDa, Observed band size: 61 kDa.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using UCKL1 Polyclonal Antibody (Product # PA5-118336) at 1:500 dilution. Lane A: 293T Whole Cell Lysate, Lane B: HeLa Whole Cell Lysate. Lysates/proteins at 30 μg per lane. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1:10,000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 61 kDa. Observed band size: 61 kDa. (We are unsure of the identity of these extra bands).

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry analysis of UCKL1 in U251MG cells. Samples were treated with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, incubated with polyclonal antibody (Product # PA5-118336) with a dilution of 1: 200 (4°C overnight), followed by Alexa Fluor 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and DAPI (blue).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence staining of UCKL1 in U251MG cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with UCKL1 Polyclonal Antibody (Product # PA5-118336, 1:200) at 4°C overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry (Paraffin) of UCKL1 in human stomach. Samples were incubated with polyclonal antibody (Product # PA5-118336) with a dilution of 1:100 .

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human UCKL1 in human stomach with UCKL1 Polyclonal Antibody (Product # PA5-118336, 1:100 dilution, formalin-fixed paraffin embedded sections).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation of UCKL1 in Lane A: 0.5 mg HeLa Whole Cell Lysate. Samples were treated with 60 μg of immunomagnetic Protein A/G beads, incubated with polyclonal antibody (Product # PA5-118336) with a dilution of 1:100 , followed by Goat Anti-Rabbit IgG (H+L)/HRP using a dilution of 1:10,000. Assay was performed under reducing conditions. Predicted band size: 61 kDa , Observed band size : 61 kDa .

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

UCKL1 Immunoprecipitation using: Lane A: 0.5 mg HeLa Whole Cell Lysate 4 µL with UCKL1 Polyclonal Antibody (Product # PA5-118336) and 60 μg of Immunomagnetic beads Protein A/G. Primary antibody: UCKL1 Polyclonal Antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L) /HRP at 1:10,000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 61 kDa. Observed band size: 61 kDa.