NBP1-42680

antibody from Novus Biologicals

Targeting: UCKL1 FLJ20517, URKL1

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP1-42680

Provider

Novus Biologicals

Proper citation

Novus Cat#NBP1-42680, RRID:AB_2210778

Product name

Rabbit Polyclonal UCKL1 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

0.1 ml

Concentration

0.2 mg/ml

Storage

Store at 4C. Do not freeze.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: UCKL1 Antibody [NBP1-42680] - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-UCKL1 antibody used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate. UCKL1 was also immunoprecipitated by rabbit anti-UCKL1 antibody NBP1-42681, which recognizes a downstream epitope.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry: UCKL1 Antibody [NBP1-42680] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-UCKL1 used at a dilution of 1:200 (1ug/ml) Detection: DAB staining using Immunohistochemistry Accessory Kit