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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunoprecipitation [1]
- Immunohistochemistry [3]
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- Product number
- NB100-79822 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-79822, RRID:AB_1107987
- Product name
- Rabbit Polyclonal CPSF1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 0.2 mg/ml
- Storage
- Store at 4C. Do not freeze.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: CPSF1 Antibody [NB100-79822] - Detection of Human and Mouse CPSF160 by Western Blot (h&m) and Immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg) and mouse NIH3T3 (M; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-CPSF160 antibody NB100-79822 used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate. CPSF160 was also immunoprecipitated by rabbit another anti-CPSF160 antibody, which recognizes an upstream epitope.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: CPSF1 Antibody [NB100-79822] - Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CPSF160 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: CPSF1 Antibody [NB100-79822] - Detection of human CPSF160 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-CPSF160 antibody NB100-79822 (lot NB100-79822-2) used for IP at 6 ug per reaction. CPSF160 was also immunoprecipitated by a previous lot of this antibody (lot NB100-79822-1). For blotting immunoprecipitated CPSF160, NB100-79822 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: CPSF1 Antibody [NB100-79822] - Human ovarian carcinoma (left) and mouse renal cell carcinoma (right). Antibody used at a dilution of 1:200 (1ug/ml).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: CPSF1 Antibody [NB100-79822] - Section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti-CPSF160 (NB100-79822). Detection: DAB
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: CPSF1 Antibody [NB100-79822] - Section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-CPSF160 (NB100-79822). Detection: DAB
