313614

antibody from BioLegend

Targeting: ITGA6 CD49f

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Recommended by provider)

Flow cytometry (Supportive data in Antibodypedia)

Blocking/Neutralizing (Recommended by provider)

Antibody Data

Product number

313614

Provider

BioLegend

Proper citation

BioLegend Cat#313614, RRID:AB_893371

Product name

LEAF™ purified anti-human/mouse CD49f [GoH3]

Antibody type

Monoclonal

Antigen

Mouse mammary tumor cells

Description

CD49f is a 120 kD integrin family member, also known as VLA-6 α chain and α6 integrin subunit. CD49f associates with either integrin β1 (CD29) or integrin β4 (CD104) to form receptors (VLA-6 or α6β4 complex) for laminin and kalinin. CD49f is expressed on platelets, monocytes, T cells, placental trophoblasts, epithelial and endothelial cells. CD49f is involved in adhesion and can act as a co-stimulatory molecule for T cell activation and proliferation. The GoH3 antibody has been reported to block laminin binding in vitro and to block integrin α6 function in vivo.

Reactivity

Human, Mouse, Bovine, Canine, Feline, Horse, Porcine, Rabbit, Sheep, Simian

Host

Rat

Conjugate

Unconjugated

Isotype

IgG

Vial size

200 µg

Concentration

1 mg/ml

Storage

The antibody solution should be stored undiluted at 4 °C. This LEAF™ solution contains no preservative; handle under aseptic conditions.

References

Submitted references

Cell signaling by urokinase-type plasminogen activator receptor induces stem cell-like properties in breast cancer cells.

Jo M, Eastman BM, Webb DL, Stoletov K, Klemke R, Gonias SL
Cancer research 2010 Nov 1;70(21):8948-58

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Reprogramming murine telomerase rapidly inhibits the growth of mouse cancer cells in vitro and in vivo.

Xu T, Xu Y, Liao CP, Lau R, Goldkorn A
Molecular cancer therapeutics 2010 Feb;9(2):438-49

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Differential requirement of mTOR in postmitotic tissues and tumorigenesis.

Nardella C, Carracedo A, Alimonti A, Hobbs RM, Clohessy JG, Chen Z, Egia A, Fornari A, Fiorentino M, Loda M, Kozma SC, Thomas G, Cordon-Cardo C, Pandolfi PP
Science signaling 2009 Jan 27;2(55):ra2

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Reduced migration, altered matrix and enhanced TGFbeta1 signaling are signatures of mouse keratinocytes lacking Sdc1.

Stepp MA, Liu Y, Pal-Ghosh S, Jurjus RA, Tadvalkar G, Sekaran A, Losicco K, Jiang L, Larsen M, Li L, Yuspa SH
Journal of cell science 2007 Aug 15;120(Pt 16):2851-63

Flow cytometry

Supportive validation

Submitted by

BioLegend (provider)

Main image

Experimental details

Human peripheral blood lymphocytes stained with LEAF™ purified GoH3, followed by anti-rat IgG FITC

Conjugate

Unconjugated