Antibody data
- Antibody Data
- Antigen structure
- References [5]
- Comments [0]
- Validations
- Western blot [5]
- Immunohistochemistry [5]
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Validation data
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- Product number
- NBP1-85747 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-85747, RRID:AB_11039415
- Product name
- Rabbit Polyclonal Integrin alpha 6/CD49f Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human Integrin alpha 6/CD49f antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references New insights into diagnosis and therapeutic options for proliferative hepatoblastoma.
Multiplex flow cytometry barcoding and antibody arrays identify surface antigen profiles of primary and metastatic colon cancer cell lines.
Tissue-engineered mucosa is a suitable model to quantify the acute biological effects of ionizing radiation.
Strain-induced differentiation of fetal type II epithelial cells is mediated via the integrin α6β1-ADAM17/tumor necrosis factor-α-converting enzyme (TACE) signaling pathway.
Identification of integrins alpha6 and beta7 as c-Jun- and transformation-relevant genes in highly invasive fibrosarcoma cells.
Hooks KB, Audoux J, Fazli H, Lesjean S, Ernault T, Dugot-Senant N, Leste-Lasserre T, Hagedorn M, Rousseau B, Danet C, Branchereau S, Brugières L, Taque S, Guettier C, Fabre M, Rullier A, Buendia MA, Commes T, Grosset CF, Raymond AA
Hepatology (Baltimore, Md.) 2018 Jul;68(1):89-102
Hepatology (Baltimore, Md.) 2018 Jul;68(1):89-102
Multiplex flow cytometry barcoding and antibody arrays identify surface antigen profiles of primary and metastatic colon cancer cell lines.
Sukhdeo K, Paramban RI, Vidal JG, Elia J, Martin J, Rivera M, Carrasco DR, Jarrar A, Kalady MF, Carson CT, Balderas R, Hjelmeland AB, Lathia JD, Rich JN
PloS one 2013;8(1):e53015
PloS one 2013;8(1):e53015
Tissue-engineered mucosa is a suitable model to quantify the acute biological effects of ionizing radiation.
Tra WM, Tuk B, van Neck JW, Hovius SE, Perez-Amodio S
International journal of oral and maxillofacial surgery 2013 Aug;42(8):939-48
International journal of oral and maxillofacial surgery 2013 Aug;42(8):939-48
Strain-induced differentiation of fetal type II epithelial cells is mediated via the integrin α6β1-ADAM17/tumor necrosis factor-α-converting enzyme (TACE) signaling pathway.
Wang Y, Huang Z, Nayak PS, Matthews BD, Warburton D, Shi W, Sanchez-Esteban J
The Journal of biological chemistry 2013 Aug 30;288(35):25646-25657
The Journal of biological chemistry 2013 Aug 30;288(35):25646-25657
Identification of integrins alpha6 and beta7 as c-Jun- and transformation-relevant genes in highly invasive fibrosarcoma cells.
Kielosto M, Nummela P, Järvinen K, Yin M, Hölttä E
International journal of cancer 2009 Sep 1;125(5):1065-73
International journal of cancer 2009 Sep 1;125(5):1065-73
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Simple Western: Integrin alpha 6/CD49f Antibody [NBP1-85747] - Simple Western lane view shows a specific band for ITGA6 in 0.2 mg/ml of RT-4 (left) and U-251MG sp (right) lysate. This experiment was performed under reducing conditions using the 66-440 kDa separation system.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Simple Western: Integrin alpha 6/CD49f Antibody [NBP1-85747] - Electropherogram image(s) of corresponding Simple Western lane view. Integrin alpha 6/CD49f antibody was used at 1:50 dilution on RT-4 and U-251MG sp lysates(s).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Integrin alpha 6/CD49f Antibody [NBP1-85747] - Analysis in human cell line HeLa.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Integrin alpha 6/CD49f Antibody [NBP1-85747] - Western blot analysis in mouse cell line NIH-3T3, rat cell line NBT-II and rat cell line pC12.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Integrin alpha 6/CD49f Antibody [NBP1-85747] - Analysis in human cell lines A-431 and MCF-7. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-GAPDH.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Integrin alpha 6/CD49f Antibody [NBP1-85747] - Staining of human placenta shows strong membranous positivity in trophoblastic cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Integrin alpha 6/CD49f Antibody [NBP1-85747] - Staining of human kidney shows moderate membranous positivity in cells in tubules.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Integrin alpha 6/CD49f Antibody [NBP1-85747] - Staining of human rectum shows strong membranous positivity in glandular cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Integrin alpha 6/CD49f Antibody [NBP1-85747] - Staining of human liver shows no positivity in hepatocytes as expected.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Integrin alpha 6/CD49f Antibody [NBP1-85747] - Staining in human placenta and liver tissues . Corresponding ITGA6 RNA-seq data are presented for the same tissues.