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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- AP12387PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AP12387PU-N, RRID:AB_1769070
- Product name
- anti CD49f / ITGA6 (Isoform 2)
- Antibody type
- Polyclonal
- Antigen
- KLH conjugated synthetic peptide between 1043-1072 amino acids from Human ITGA6.
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 0.4 ml
- Concentration
- lot specific
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot analysis of anti-ITGA6 Antibody (Isoform 2) (Cat.#AP12387PU-N) in 293 cell line lysates (35ug/lane). ITGA6 (arrow) was detected using the purified Pab.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Fluorescent confocal image of HeLa cells stained with ITGA6 Antibody (isoform 2) (Cat.#AP12387PU-N). HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AP12387PU-N ITGA6 (isoform 2 S1064) primary antibody (1/200, 2 h at room temperature). For Secondary antibody, Alexa Fluor®488 conjugated Donkey anti-Rabbit antibody (green) was used (1/1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/ml, 5 min). Note the highly specific localization of the ITGA6 mainly to the nucleus, supported by Human Protein Atlas Data (http://www.proteinatlas.org/ENSG00000091409).
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human colon carcinoma tissue reacted with ITGA6 Antibody (isoform 2) (Cat.#AP12387PU-N), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
