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LearnPA5-20859
antibody from Invitrogen Antibodies
Targeting: TMEM38B
bA219P18.1, C9orf87, D4Ertd89e, FLJ10493, TRIC-B
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [2]
- Other assay [1]
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- Product number
- PA5-20859 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TMEM38B Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- A suggested positive control is rat thymus tissue lysate. PA5-20859 can be used with blocking peptide PEP-0973.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C
Submitted references TRIC-A shapes oscillatory Ca2+ signals by interaction with STIM1/Orai1 complexes.
XBP1-KLF9 Axis Acts as a Molecular Rheostat to Control the Transition from Adaptive to Cytotoxic Unfolded Protein Response.
Absence of the ER Cation Channel TMEM38B/TRIC-B Disrupts Intracellular Calcium Homeostasis and Dysregulates Collagen Synthesis in Recessive Osteogenesis Imperfecta.
Shrestha N, Bacsa B, Ong HL, Scheruebel S, Bischof H, Malli R, Ambudkar IS, Groschner K
PLoS biology 2020 Apr;18(4):e3000700
PLoS biology 2020 Apr;18(4):e3000700
XBP1-KLF9 Axis Acts as a Molecular Rheostat to Control the Transition from Adaptive to Cytotoxic Unfolded Protein Response.
Fink EE, Moparthy S, Bagati A, Bianchi-Smiraglia A, Lipchick BC, Wolff DW, Roll MV, Wang J, Liu S, Bakin AV, Kandel ES, Lee AH, Nikiforov MA
Cell reports 2018 Oct 2;25(1):212-223.e4
Cell reports 2018 Oct 2;25(1):212-223.e4
Absence of the ER Cation Channel TMEM38B/TRIC-B Disrupts Intracellular Calcium Homeostasis and Dysregulates Collagen Synthesis in Recessive Osteogenesis Imperfecta.
Cabral WA, Ishikawa M, Garten M, Makareeva EN, Sargent BM, Weis M, Barnes AM, Webb EA, Shaw NJ, Ala-Kokko L, Lacbawan FL, Högler W, Leikin S, Blank PS, Zimmerberg J, Eyre DR, Yamada Y, Marini JC
PLoS genetics 2016 Jul;12(7):e1006156
PLoS genetics 2016 Jul;12(7):e1006156
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of rat thymus tissue lysate using a TMEM38B polyclonal antibody (Product # PA5-20859) at (A) 1 and (B) 2 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of TMEM38B in rat thymus tissue lysate with TMEM38B Polyclonal Antibody (Product # PA5-20859) at (A) 1 and (B) 2 µg/mL.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence of TMEM38B in mouse thymus tissue with TMEM38B Polyclonal Antibody (Product # PA5-20859) at 20 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of TMEM38B in mouse thymus tissue with TMEM38B Polyclonal Antibody (Product # PA5-20859) at 5 µg/mL.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig 2 The TMEM38B mutations result in alleles. (A) Real-time RT-PCR analysis of TMEM38B transcripts from control (C) and proband (P1, P2 and P3) fibroblasts and osteoblasts. Rescue of transcripts by treatment of cells with emetine (+) indicates inhibition of nonsense-mediated decay due to the presence of premature termination codons (PTCs). **, p < 0.01. (B) RT-PCR analysis of TMEM38B mRNA from control (C) and P1 (OI) fibroblasts in the absence or presence emetine. Several unique amplification products were observed for P1 samples, including multiple splice variants and heteroduplexes (HD), as detailed in S1 Fig . (C) Western analysis of control (C) and proband (P1, P2 and P3) fibroblast and osteoblast lysates indicates absence of TRIC-B protein in proband cells.
