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Western blot
Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
- References [5]
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- Validations
- Blocking/Neutralizing [1]
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- Product number
- MAB528-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse CCL24/Eotaxin-2/MPIF-2 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects mouse CCL24/Eotaxin-2/MPIF-2 in Western blots. In Western blots, no cross-reactivity with recombinant human CCL1, 2, 3, 4, 5, 7, 8, 11, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 28, recombinant mouse CCL1, 2, 3, 4, 6, 7, 9/10/MIP-1 gamma , 11, 12, 19, 20, 21, 22, 25, 28, or recombinant rat CCL20 is observed.
- Reactivity
- Mouse
- Host
- Rat
- Conjugate
- Unconjugated
- Antigen sequence
Q9JKC0- Isotype
- IgG
- Antibody clone number
- 106521
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references A critical role for IRF5 in regulating allergic airway inflammation.
TPL-2 restricts Ccl24-dependent immunity to Heligmosomoides polygyrus.
Pulmonary Epithelial Cell-Derived Cytokine TGF-β1 Is a Critical Cofactor for Enhanced Innate Lymphoid Cell Function.
IL-23 and Th17 cells enhance Th2-cell-mediated eosinophilic airway inflammation in mice.
Intranasal mite allergen induces allergic asthma-like responses in NC/Nga mice.
Byrne AJ, Weiss M, Mathie SA, Walker SA, Eames HL, Saliba D, Lloyd CM, Udalova IA
Mucosal immunology 2017 May;10(3):716-726
Mucosal immunology 2017 May;10(3):716-726
TPL-2 restricts Ccl24-dependent immunity to Heligmosomoides polygyrus.
Kannan Y, Entwistle LJ, Pelly VS, Perez-Lloret J, Walker AW, Ley SC, Wilson MS
PLoS pathogens 2017 Jul;13(7):e1006536
PLoS pathogens 2017 Jul;13(7):e1006536
Pulmonary Epithelial Cell-Derived Cytokine TGF-β1 Is a Critical Cofactor for Enhanced Innate Lymphoid Cell Function.
Denney L, Byrne AJ, Shea TJ, Buckley JS, Pease JE, Herledan GM, Walker SA, Gregory LG, Lloyd CM
Immunity 2015 Nov 17;43(5):945-58
Immunity 2015 Nov 17;43(5):945-58
IL-23 and Th17 cells enhance Th2-cell-mediated eosinophilic airway inflammation in mice.
Wakashin H, Hirose K, Maezawa Y, Kagami S, Suto A, Watanabe N, Saito Y, Hatano M, Tokuhisa T, Iwakura Y, Puccetti P, Iwamoto I, Nakajima H
American journal of respiratory and critical care medicine 2008 Nov 15;178(10):1023-32
American journal of respiratory and critical care medicine 2008 Nov 15;178(10):1023-32
Intranasal mite allergen induces allergic asthma-like responses in NC/Nga mice.
Shibamori M, Ogino K, Kambayashi Y, Ishiyama H
Life sciences 2006 Jan 25;78(9):987-94
Life sciences 2006 Jan 25;78(9):987-94
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Supportive validation
- Submitted by
- R&D Systems (provider)
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- Experimental details
- Chemotaxis Induced by CCL24/Eotaxin-2 and Neutral-ization by Mouse CCL24/ Eotaxin-2 Antibody. Recombinant Mouse CCL24/ Eotaxin-2 (Catalog # 1017-MP) chemoattracts the BaF3 mouse pro-B cell line transfected with mouse CCR3 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CCL24/Eotaxin-2 (20 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse CCL24/Eotaxin-2 Monoclonal Antibody (Catalog # MAB528). The ND50 is typically 0.06-0.3 µg/mL.
