Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Immunohistochemistry [1]
- Blocking/Neutralizing [1]
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Validation data
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- Product number
- AF343 - Provider product page
- Provider
- R&D Systems
- Product name
- Human CCL24/Eotaxin-2/MPIF-2 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human CCL24/Eotaxin-2/MPIF-2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 10% cross-reactivity with recombinant mouse CCL24/Eotaxin-2/MPIF-2 is observed and less than 5% cross-reactivity with recombinant human (rh) MPIF-1 is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
AAB51135
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references An initial investigation into endothelial CC chemokine expression in the human rheumatoid synovium.
Mast cell beta-tryptase selectively cleaves eotaxin and RANTES and abrogates their eosinophil chemotactic activities.
Mast cell beta-tryptase selectively cleaves eotaxin and RANTES and abrogates their eosinophil chemotactic activities.
Rump L, Mattey DL, Kehoe O, Middleton J
Cytokine 2017 Sep;97:133-140
Cytokine 2017 Sep;97:133-140
Mast cell beta-tryptase selectively cleaves eotaxin and RANTES and abrogates their eosinophil chemotactic activities.
Pang L, Nie M, Corbett L, Sutcliffe A, Knox AJ
Journal of immunology (Baltimore, Md. : 1950) 2006 Mar 15;176(6):3788-95
Journal of immunology (Baltimore, Md. : 1950) 2006 Mar 15;176(6):3788-95
Mast cell beta-tryptase selectively cleaves eotaxin and RANTES and abrogates their eosinophil chemotactic activities.
Pang L, Nie M, Corbett L, Sutcliffe A, Knox AJ
Journal of immunology (Baltimore, Md. : 1950) 2006 Mar 15;176(6):3788-95
Journal of immunology (Baltimore, Md. : 1950) 2006 Mar 15;176(6):3788-95
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- CCL24/Eotaxin-2/MPIF-2 in Human Skin. CCL24/Eotaxin-2/MPIF-2 was detected in immersion fixed paraffin-embedded sections of human skin using 15 µg/mL Goat Anti-Human CCL24/Eotaxin-2/ MPIF-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF343) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Chemotaxis Induced by CCL24/Eotaxin-2 and Neutralization by Human CCL24/Eotaxin-2 Antibody. Recombinant Human CCL24/Eotaxin-2 (Catalog # 343-E2) chemoattracts the BaF3 mouse pro-B cell line transfected with mouse CCR3 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL24/Eotaxin-2 (0.1 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human CCL24/Eotaxin-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF343). The ND50 is typically 0.5-2.5 µg/mL.