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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [3]
- Immunohistochemistry [1]
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- Product number
- A303-270A - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ZNF462 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- 4° C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human ZNF462 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ZNF462 antibody A303-270A (lot A303-270A-2) used for IP at 6 µg per reaction. ZNF462 was also immunoprecipitated by a previous lot of this antibody A303-270A-1. For blotting immunoprecipitated ZNF462, A303-270A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of mouse ZNF462 by western blot. Samples: Whole cell lysate (50 µg) from NIH3T3 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ZNF462 antibody A303-270A (lot A303-270A-2) used for WB at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human ZNF462 by western blot. Samples: Whole cell lysate (50 µg) from HeLa and 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ZNF462 antibody A303-270A (lot A303-270A-2) used for WB at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human ZNF462 by immunohistochemistry. Sample: FFPE section of human lung cancer. Antibody: Affinity purified rabbit anti- ZNF462 (Cat. No. A303-270A Lot2) used at a dilution of 1:5,000 (0.2µg/ml). Detection: DAB.
