CABT-B9367

antibody from Creative Diagnostics

Targeting: ARHGEF7 BETA-PIX, COOL1, DKFZp686C12170, DKFZp761K1021, KIAA0142, Nbla10314, P50, P50BP, P85, P85COOL1, P85SPR, PAK3, PIXB

Western blot (Recommended by provider)

Immunocytochemistry (Recommended by provider)

Antibody Data

Product number

CABT-B9367

Provider

Creative Diagnostics

Product name

Mouse anti-Rat ÉÇPIX monoclonal antibody, clone 34/cQJY

Antibody type

Monoclonal

Description

The activity of PAK family kinases is regulated through interaction with the small GTPases Cdc42 and Rac1. PAKs are activated by the GTP-bound form of Cdc-42 and Rac1, and recruitment of PAKs to focal complexes has been implicated in Cdc42- and Rac1-dependent regulation of focal contact formation. P AK- i nteracting e x change factor (PIX) was identified in a screen for proteins that bind PAKs. Two forms of PIX have been identified: an 85 kDa protein designated Ï« PIX and a 78 kDa protein designated ÉÇ PIX. These proteins have 80% identity in their overlapping regions, which include myosin-like, pleckstrin (PH), Dbl (DH), and SH3 domains. In addition, Ï« PIX contains a calponin-like domain at the N-terminus. The expression of ÉÇ PIX is ubiquitous, while Ï« PIX is expressed in heart, muscle, and thymus. PIX can act as a guanine nuleotide exchange factor for Rac1 and co-transfection of ÉÇ PIX, Cdc42, and Ï« PAK results in increased Ï« PAK activity. PIX binding to PAK is required for localization of PAKs to focal complexes and injection of ÉÇ PIX leads to Rac1-dependent membrane ruffling. Thus, PIX is important for PAK localization and activity during small GTPase-dependent regulation of cell morphology.

Reactivity

Mouse, Rat, Canine, Chicken/Avian

Host

Mouse

Conjugate

Unconjugated

Isotype

IgG

Antibody clone number

34/cQJY

Vial size

50 _g

Concentration

250 Ã×g/ml

Storage

Store undiluted at -20°C.