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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- AP17091PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AP17091PU-N, RRID:AB_10678449
- Product name
- anti Alcohol dehydrogenase 1B (ADH2)
- Antibody type
- Polyclonal
- Antigen
- KLH conjugated synthetic peptide between 216~245 amino acids from the Center region of Human ADH1B
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 0.4 ml
- Concentration
- lot specific
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot analysis of ADH1B Antibody (Center) Cat.-No AP17091PU-N in HepG2 cell line lysates (35 µg/lane). ADH1B (arrow) was detected using the purified Pab.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Confocal immunofluorescent analysis of ADH1B Antibody (Center)Cat.-No AP17091PU-N with HepG2 cell followed by Alexa Fluor® 488-conjugated Goat anti-Rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with ADH1B Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Flow Cytometry analysis of HepG2 cells using ADH1B Antibody (Center) Cat.-No AP17091PU-N (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
