NBP2-00649

antibody from Novus Biologicals

Targeting: ADH1B ADH2

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP2-00649

Provider

Novus Biologicals

Product name

Mouse Monoclonal alcohol dehydrogenase 1B Antibody

Antibody type

Monoclonal

Description

Affinity purified.

Reactivity

Human, Rat, Canine

Host

Mouse

Isotype

IgG

Vial size

0.1 ml

Concentration

1 mg/ml

Storage

Store at -20C. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: alcohol dehydrogenase 1B Antibody (OTI4F12) [NBP2-00649] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY alcohol dehydrogenase 1B (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-alcohol dehydrogenase 1B.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: alcohol dehydrogenase 1B Antibody (OTI4F12) [NBP2-00649] - Analysis of extracts (35ug) from 9 different cell lines by using anti-alcohol dehydrogenase 1B monoclonal antibody.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: alcohol dehydrogenase 1B Antibody (OTI4F12) [NBP2-00649] - Analysis of extracts (10ug) from 9 Human tissue by using anti-ADH1B monoclonal antibody at 1:1000 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon).

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: alcohol dehydrogenase 1B Antibody (OTI4F12) [NBP2-00649] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-alcohol dehydrogenase 1B mouse monoclonal antibody.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: alcohol dehydrogenase 1B Antibody (OTI4F12) [NBP2-00649] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-alcohol dehydrogenase 1B mouse monoclonal antibody.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: alcohol dehydrogenase 1B Antibody (OTI4F12) [NBP2-00649] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-alcohol dehydrogenase 1B mouse monoclonal antibody.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: alcohol dehydrogenase 1B Antibody (OTI4F12) [NBP2-00649] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-alcohol dehydrogenase 1B mouse monoclonal antibody.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: alcohol dehydrogenase 1B Antibody (OTI4F12) [NBP2-00649] - Staining of paraffin-embedded Human liver tissue using anti-alcohol dehydrogenase 1B mouse monoclonal antibody.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: alcohol dehydrogenase 1B Antibody (OTI4F12) [NBP2-00649] - Staining of paraffin-embedded Human lung tissue using anti-alcohol dehydrogenase 1B mouse monoclonal antibody.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: alcohol dehydrogenase 1B Antibody (OTI4F12) [NBP2-00649] - Staining of paraffin-embedded Human lymph node tissue using anti-alcohol dehydrogenase 1B mouse monoclonal antibody.

Flow cytometry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: alcohol dehydrogenase 1B Antibody (OTI4F12) [NBP2-00649] - Analysis of Hela cells, using anti-alcohol dehydrogenase 1B antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: alcohol dehydrogenase 1B Antibody (OTI4F12) [NBP2-00649] - Analysis of Jurkat cells, using anti-alcohol dehydrogenase 1B antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: alcohol dehydrogenase 1B Antibody (OTI4F12) [NBP2-00649] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-alcohol dehydrogenase 1B antibody, and then analyzed by flow cytometry.