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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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- Product number
- GTX102237 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX102237, RRID:AB_1950338
- Product name
- alpha Glucosidase II antibody [N1N2], N-term
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse
- Host
- Rabbit
Submitted references Genetic dissection of mammalian ERAD through comparative haploid and CRISPR forward genetic screens.
Timms RT, Menzies SA, Tchasovnikarova IA, Christensen LC, Williamson JC, Antrobus R, Dougan G, Ellgaard L, Lehner PJ
Nature communications 2016 Jun 10;7:11786
Nature communications 2016 Jun 10;7:11786
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (50 ?g of whole cell lysate) A: Mouse brain 7.5% SDS PAGE GTX102237 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample(30 ?g whole cell lysate) A: 293TB: A431 (GTX27909)C: H1299D: HeLa S3 (GTX14654)E: HepG2 (GTX27900)F: MOLT4 (GTX27912)G: Raji (GTX27908)7.5% SDS PAGEGTX102237 diluted at 1:1000The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using alpha Glucosidase II(GTX102237) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded SW480 xenograft , using alpha Glucosidase II(GTX102237) antibody at 1:500 dilution.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- alpha Glucosidase II antibody [N1N2], N-term detects alpha Glucosidase II protein at cytoplasm in human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. alpha Glucosidase II antibody [N1N2], N-term (GTX102237) diluted at 1:500.
