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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PAB6584 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB6584, RRID:AB_1581871
- Product name
- VPS28 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Goat polyclonal antibody raised against synthetic peptide of VPS28.
- Antigen sequence
C-ESAYNAFNRFLHA- Storage
- Store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references TSG101/mammalian VPS23 and mammalian VPS28 interact directly and are recruited to VPS4-induced endosomes.
Bishop N, Woodman P
The Journal of biological chemistry 2001 Apr 13;276(15):11735-42
The Journal of biological chemistry 2001 Apr 13;276(15):11735-42
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- VPS28 polyclonal antibody (Cat # PAB6584) staining (1 ug/mL) of human testis lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- VPS28 polyclonal antibody (Cat # PAB6584) (10 ug/mL) staining of paraffin embedded human testis. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
