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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- ELISA [1]
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- Product number
- H00002889-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00002889-M01, RRID:AB_547590
- Product name
- Mouse Monoclonal GRF2 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. RAPGEF1 - Rap guanine nucleotide exchange factor (GEF) 1
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references The WAVE2 complex regulates T cell receptor signaling to integrins via Abl- and CrkL-C3G-mediated activation of Rap1.
Nolz JC, Nacusi LP, Segovis CM, Medeiros RB, Mitchell JS, Shimizu Y, Billadeau DD
The Journal of cell biology 2008 Sep 22;182(6):1231-44
The Journal of cell biology 2008 Sep 22;182(6):1231-44
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: GRF2 Antibody (3D10) [H00002889-M01] - Analysis of RAPGEF1 expression in transfected 293T cell line by RAPGEF1 monoclonal antibody (M01), clone 3D10.Lane 1: RAPGEF1 transfected lysate(122.7 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Sandwich ELISA: GRF2 Antibody (3D10) [H00002889-M01] - Detection limit for recombinant GST tagged RAPGEF1 is approximately 0.1ng/ml as a capture antibody.
