Antibody data
- Antibody Data
- Antigen structure
- References [14]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Chromatin Immunoprecipitation [1]
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Validation data
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- Product number
- AF3640 - Provider product page
- Provider
- R&D Systems
- Product name
- Human KLF4 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human KLF4 in direct ELISAs and Western blots. In direct ELISAs, this antibody shows less than 30% cross-reactivity with recombinant mouse KLF4.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
AAH29923
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Measles vector as a multigene delivery platform facilitating iPSC reprogramming.
Hominoid-Specific Transposable Elements and KZFPs Facilitate Human Embryonic Genome Activation and Control Transcription in Naive Human ESCs.
Kruppel-like factor 4 regulates keratinocyte senescence.
AAVvector-mediated in vivo reprogramming into pluripotency.
Data in support of sustained upregulation of adaptive redox homeostasis mechanisms caused by KRIT1 loss-of-function.
Cancer stem-like cells can be induced through dedifferentiation under hypoxic conditions in glioma, hepatoma and lung cancer.
KLF2 and KLF4 control endothelial identity and vascular integrity.
HIF1α regulates single differentiated glioma cell dedifferentiation to stem-like cell phenotypes with high tumorigenic potential under hypoxia.
Induced overexpression of Oct4A in human dental pulp cells enhances pluripotency and multilineage differentiation capability.
Lymphatic regulator PROX1 determines Schlemm's canal integrity and identity.
ZNF750 interacts with KLF4 and RCOR1, KDM1A, and CTBP1/2 chromatin regulators to repress epidermal progenitor genes and induce differentiation genes.
Endogenous purification reveals GREB1 as a key estrogen receptor regulatory factor.
KLF4 modulates expression of IL-6 in dendritic cells via both promoter activation and epigenetic modification.
Innate immune suppression enables frequent transfection with RNA encoding reprogramming proteins.
Wang Q, Vossen A, Ikeda Y, Devaux P
Gene therapy 2019 May;26(5):151-164
Gene therapy 2019 May;26(5):151-164
Hominoid-Specific Transposable Elements and KZFPs Facilitate Human Embryonic Genome Activation and Control Transcription in Naive Human ESCs.
Pontis J, Planet E, Offner S, Turelli P, Duc J, Coudray A, Theunissen TW, Jaenisch R, Trono D
Cell stem cell 2019 May 2;24(5):724-735.e5
Cell stem cell 2019 May 2;24(5):724-735.e5
Kruppel-like factor 4 regulates keratinocyte senescence.
Panatta E, Lena AM, Mancini M, Affinati M, Smirnov A, Annicchiarico-Petruzzelli M, Piro MC, Campione E, Bianchi L, Mazzanti C, Melino G, Candi E
Biochemical and biophysical research communications 2018 May 5;499(2):389-395
Biochemical and biophysical research communications 2018 May 5;499(2):389-395
AAVvector-mediated in vivo reprogramming into pluripotency.
Senís E, Mosteiro L, Wilkening S, Wiedtke E, Nowrouzi A, Afzal S, Fronza R, Landerer H, Abad M, Niopek D, Schmidt M, Serrano M, Grimm D
Nature communications 2018 Jul 9;9(1):2651
Nature communications 2018 Jul 9;9(1):2651
Data in support of sustained upregulation of adaptive redox homeostasis mechanisms caused by KRIT1 loss-of-function.
Antognelli C, Trapani E, Delle Monache S, Perrelli A, Fornelli C, Retta F, Cassoni P, Talesa VN, Retta SF
Data in brief 2018 Feb;16:929-938
Data in brief 2018 Feb;16:929-938
Cancer stem-like cells can be induced through dedifferentiation under hypoxic conditions in glioma, hepatoma and lung cancer.
Wang P, Wan WW, Xiong SL, Feng H, Wu N
Cell death discovery 2017;3:16105
Cell death discovery 2017;3:16105
KLF2 and KLF4 control endothelial identity and vascular integrity.
Sangwung P, Zhou G, Nayak L, Chan ER, Kumar S, Kang DW, Zhang R, Liao X, Lu Y, Sugi K, Fujioka H, Shi H, Lapping SD, Ghosh CC, Higgins SJ, Parikh SM, Jo H, Jain MK
JCI insight 2017 Feb 23;2(4):e91700
JCI insight 2017 Feb 23;2(4):e91700
HIF1α regulates single differentiated glioma cell dedifferentiation to stem-like cell phenotypes with high tumorigenic potential under hypoxia.
Wang P, Lan C, Xiong S, Zhao X, Shan Y, Hu R, Wan W, Yu S, Liao B, Li G, Wang J, Zou D, Chen B, Feng H, Wu N
Oncotarget 2017 Apr 25;8(17):28074-28092
Oncotarget 2017 Apr 25;8(17):28074-28092
Induced overexpression of Oct4A in human dental pulp cells enhances pluripotency and multilineage differentiation capability.
Liu L, Wu L, Wei X, Ling J
Stem cells and development 2015 Apr 15;24(8):962-72
Stem cells and development 2015 Apr 15;24(8):962-72
Lymphatic regulator PROX1 determines Schlemm's canal integrity and identity.
Park DY, Lee J, Park I, Choi D, Lee S, Song S, Hwang Y, Hong KY, Nakaoka Y, Makinen T, Kim P, Alitalo K, Hong YK, Koh GY
The Journal of clinical investigation 2014 Sep;124(9):3960-74
The Journal of clinical investigation 2014 Sep;124(9):3960-74
ZNF750 interacts with KLF4 and RCOR1, KDM1A, and CTBP1/2 chromatin regulators to repress epidermal progenitor genes and induce differentiation genes.
Boxer LD, Barajas B, Tao S, Zhang J, Khavari PA
Genes & development 2014 Sep 15;28(18):2013-26
Genes & development 2014 Sep 15;28(18):2013-26
Endogenous purification reveals GREB1 as a key estrogen receptor regulatory factor.
Mohammed H, D'Santos C, Serandour AA, Ali HR, Brown GD, Atkins A, Rueda OM, Holmes KA, Theodorou V, Robinson JL, Zwart W, Saadi A, Ross-Innes CS, Chin SF, Menon S, Stingl J, Palmieri C, Caldas C, Carroll JS
Cell reports 2013 Feb 21;3(2):342-9
Cell reports 2013 Feb 21;3(2):342-9
KLF4 modulates expression of IL-6 in dendritic cells via both promoter activation and epigenetic modification.
Rosenzweig JM, Glenn JD, Calabresi PA, Whartenby KA
The Journal of biological chemistry 2013 Aug 16;288(33):23868-74
The Journal of biological chemistry 2013 Aug 16;288(33):23868-74
Innate immune suppression enables frequent transfection with RNA encoding reprogramming proteins.
Angel M, Yanik MF
PloS one 2010 Jul 23;5(7):e11756
PloS one 2010 Jul 23;5(7):e11756
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human KLF4 by Western Blot. Western blot shows lysates of HT-29 human colon adenocarcinoma cell line, SW480 human colorectal adenocarcinoma cell line, and HCT-116 human colorectal carcinoma cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human KLF4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3640) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for KLF4 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 5.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human KLF4 by Simple WesternTM. Simple Western lane view shows lysates of HT-29 human colon adenocarcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for KLF4 at approximately 63 kDa (as indicated) using 25 µg/mL of Goat Anti-Human KLF4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3640) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- KLF4 in BG01V Human Stem Cells. KLF4 was detected in immersion fixed BG01V human embryonic stem cells using 10 µg/mL Goat Anti-Human KLF4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3640) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- KLF4 in Human Colon. KLF4 was detected in immersion fixed paraffin-embedded sections of human colon using 15 µg/mL Goat Anti-Human KLF4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3640) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the nuclei of epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of KLF4-regulated Genes by Chromatin Immunoprecipitation. BG01V human embryonic stem cells were fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. KLF4/DNA complexes were immunoprecipitated using 5 μg Goat Anti-Human KLF4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3640) or control antibody (Catalog # AB-108-C) for 15 minutes in an ultrasonic bath, followed by Biotinylated Anti-Goat IgG Secondary Antibody (Catalog # BAF109). Immunocomplexes were captured using 50 μL of MagCellect Streptavidin Ferrofluid (Catalog # MAG999) and DNA was purified using chelating resin solution. The B2R promoter was detected by standard PCR.