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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [4]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- 12262-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ETFA Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: MFRAAAPGQ LRRAASLLRF QSTLVIAEHA NDSLAPITLN TITAATRLGG EVSCLVAGTK CDKVAQDLCK VAGIAKVLVA QHDVYKGLLP EELTPLILAT QKQFNYTHIC AGASAFGKNL LPRVAAKLEV APISDIIAIK SPDTFVRTIY AGNALCTVKC DEKVKVFSVR GTSFDAAATS GGSASSEKAS STSPVEISEW LDQKLTKSDR PELTGAKVVV SGGRGLKSGE NFKLLYDLAD QLHAAVGASR AAVDAGFVPN DMQVGQTGKI VAPELYIAVG ISGAIQHLAG MKDSKTIVAI NKDPEAPIFQ VADYGIVADL FKVVPEMTEI LKKK (1-333 aa encoded by BC015526)
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.19 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Human ileum tissue were subjected to SDS PAGE followed by western blot with 12262-1-AP (ETFA antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Y79 cells were subjected to SDS PAGE followed by western blot with 12262-1-AP (ETFA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Y79 cells were subjected to SDS PAGE followed by western blot with 12262-1-AP (ETFA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 12262-1-AP (ETFA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of MCF-7 cells, using PNRC2 antibody 12262-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human breast cancer using 12262-1-AP (ETFA antibody) at dilution of 1:50 (under 40x lens).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- 1X10^6 MCF-7 cells were stained with 0.2ug ETFA antibody (12262-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L) with dilution 1:1500.
