NBP1-32259

antibody from Novus Biologicals

Targeting: MDH2

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP1-32259

Provider

Novus Biologicals

Proper citation

Novus Cat#NBP1-32259, RRID:AB_2143289

Product name

Rabbit Polyclonal MDH2 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

0.1 ml

Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: MDH2 Antibody [NBP1-32259] - Analysis of MDH2 in HeLa whole cell lysate. Image courtesy of anonymous customer product review.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: MDH2 Antibody [NBP1-32259] - Sample (20 ug of whole cell lysate) A: mouse brain 10% SDS PAGE diluted at 1:10000

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: MDH2 Antibody [NBP1-32259] - A. 50 ug rat lung lysate/extract 10 % SDS-PAGE MDH2 antibody dilution: 1:5000

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: MDH2 Antibody [NBP1-32259] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with MDH2 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: MDH2 Antibody [NBP1-32259] - Wild-type (WT) and MDH2 knockout (KO) 293T cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with MDH2 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: MDH2 Antibody [NBP1-32259] - Paraffin-embedded OVCA xenograft.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: MDH2 Antibody [NBP1-32259] - Paraffin-embedded mouse kidney. MDH2 antibody diluted at 1:500.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: MDH2 Antibody [NBP1-32259] - Paraffin-embedded mouse heart. MDH2 antibody diluted at 1:500.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: MDH2 Antibody [NBP1-32259] - MDH2 antibody detects MDH2 protein at mitochondria by immunohistochemical analysis. Sample: Paraffin-embedded rat liver. MDH2 stained by MDH2 antibody diluted at 1:1000. Antigen Retrieval: Tris-EDTA buffer, pH 9.0, 15 min

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: MDH2 Antibody [NBP1-32259] - MDH2 antibody detects MDH2 protein at mitochondria by immunohistochemical analysis. Sample: Paraffin-embedded rat testis. MDH2 stained by MDH2 antibody diluted at 1:1000. Antigen Retrieval: Tris-EDTA buffer, pH 9.0, 15 min

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: MDH2 Antibody [NBP1-32259] - MDH2 antibody detects MDH2 protein at mitochondria by immunohistochemical analysis. Sample: Paraffin-embedded mouse testis. MDH2 stained by MDH2 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: MDH2 Antibody [NBP1-32259] - MDH2 antibody detects MDH2 protein at mitochondria by immunohistochemical analysis. Sample: Paraffin-embedded rat colon. MDH2 stained by MDH2 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.