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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Other assay [1]
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Validation data
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- Product number
- MA5-14982 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- VASP Monoclonal Antibody (G.903.3)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Description
- It is not recommended to aliquot this antibody.
- Reactivity
- Human, Mouse, Rat, Bovine, Hamster
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- G.903.3
- Vial size
- 100 µL
- Concentration
- 62 µg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using VASP Monoclonal Antibody (G.903.3) (Product # MA5-14982) and a 50 kDa band corresponding to VASP was observed in HEL92.1.7, A431, HeLa, Mouse Spleen and Mouse Lung along with an uncharacterized band (*) at ~40 kDa; and was absent in HEK293, SHSY5Y, Mouse Brain, Mouse Cerebellum and Mouse Skeletal Muscle. Whole cell extracts (30 µg lysate) of HEL 92.1.7 (Lane 1), A431 (Lane 2), HEK-293 (Lane 3), HeLa (Lane 4), SHSY5Y (Lane 5), tissue extracts (30 µg lysate) of Mouse Spleen (Lane 6), Mouse Lung (Lane 7), Mouse Brain (Lane 8), Mouse Cerebellum (Lane 9) and Mouse Skeletal Muscle (Lane 10) were electrophoresed using NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001).The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of VASP in HUVE cells using a VASP monoclonal antibody (Product # MA5-14982) (green) and a MEK1/2 monoclonal antibody (red). DNA is labeled using a fluorescent blue dye.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of VASP from HeLa cell extracts using a VASP monoclonal antibody (Product # MA5-14982) followed by Western blot analysis using the same antibody.
