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Western blot
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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [5]
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- Validations
- Immunocytochemistry [1]
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- Product number
- ALX-804-240-C100 - Provider product page
- Provider
- Enzo Life Sciences
- Proper citation
- Enzo Life Sciences Cat#ALX-804-240-C100, RRID:AB_11179348
- Product name
- [pSer239]VASP monoclonal antibody (16C2)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Description
- Thiophilic adsorption and size exclusion chromatography purified.
- Reactivity
- Human, Mouse, Rat, Rabbit
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 16C2
- Vial size
- 100 μg
- Storage
- -20°C
- Handling
- Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and freeze in liquid nitrogen.
Submitted references Cyclic nucleotides modulate store-mediated calcium entry through the activation of protein-tyrosine phosphatases and altered actin polymerization in human platelets.
Effects of in vivo nitroglycerin treatment on activity and expression of the guanylyl cyclase and cGMP-dependent protein kinase and their downstream target vasodilator-stimulated phosphoprotein in aorta.
KT5823 inhibits cGMP-dependent protein kinase activity in vitro but not in intact human platelets and rat mesangial cells.
Flow cytometry analysis of intracellular VASP phosphorylation for the assessment of activating and inhibitory signal transduction pathways in human platelets--definition and detection of ticlopidine/clopidogrel effects.
Analysis and regulation of vasodilator-stimulated phosphoprotein serine 239 phosphorylation in vitro and in intact cells using a phosphospecific monoclonal antibody.
Rosado JA, Porras T, Conde M, Sage SO
The Journal of biological chemistry 2001 May 11;276(19):15666-75
The Journal of biological chemistry 2001 May 11;276(19):15666-75
Effects of in vivo nitroglycerin treatment on activity and expression of the guanylyl cyclase and cGMP-dependent protein kinase and their downstream target vasodilator-stimulated phosphoprotein in aorta.
Mülsch A, Oelze M, Klöss S, Mollnau H, Töpfer A, Smolenski A, Walter U, Stasch JP, Warnholtz A, Hink U, Meinertz T, Münzel T
Circulation 2001 May 1;103(17):2188-94
Circulation 2001 May 1;103(17):2188-94
KT5823 inhibits cGMP-dependent protein kinase activity in vitro but not in intact human platelets and rat mesangial cells.
Burkhardt M, Glazova M, Gambaryan S, Vollkommer T, Butt E, Bader B, Heermeier K, Lincoln TM, Walter U, Palmetshofer A
The Journal of biological chemistry 2000 Oct 27;275(43):33536-41
The Journal of biological chemistry 2000 Oct 27;275(43):33536-41
Flow cytometry analysis of intracellular VASP phosphorylation for the assessment of activating and inhibitory signal transduction pathways in human platelets--definition and detection of ticlopidine/clopidogrel effects.
Schwarz UR, Geiger J, Walter U, Eigenthaler M
Thrombosis and haemostasis 1999 Sep;82(3):1145-52
Thrombosis and haemostasis 1999 Sep;82(3):1145-52
Analysis and regulation of vasodilator-stimulated phosphoprotein serine 239 phosphorylation in vitro and in intact cells using a phosphospecific monoclonal antibody.
Smolenski A, Bachmann C, Reinhard K, Hönig-Liedl P, Jarchau T, Hoschuetzky H, Walter U
The Journal of biological chemistry 1998 Aug 7;273(32):20029-35
The Journal of biological chemistry 1998 Aug 7;273(32):20029-35
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- Enzo Life Sciences (provider)
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- Experimental details
- Western blot analysis to illustrate phosphospecificity.Whole cell extracts of control (co) or Forskolin (F) treated MDA-MB-231 tumor cells were applied to SDS-PAGE (ca. 20'000 cells per lane) and transfereed to PVDF membranes. Immunoblots were probed with MAb to VASP (phosphorylated) (pSer239) (16C2) (Prod. No. ALX-804-240) for 1h at RT and developed by ECL (exp. time: 30 s).
