AF5535

antibody from R&D Systems

Targeting: SGPL1 SPL

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

AF5535

Provider

R&D Systems

Product name

Human SGPL1 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Detects human SGPL1 in direct ELISAs and Western blots.

Reactivity

Human

Host

Goat

Conjugate

Unconjugated

Antigen sequence

O95470


Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

References

Submitted references

Nicotinamide phosphoribosyltransferase is a molecular target of potent anticancer agents identified from phenotype-based drug screening.

Yamaguchi D, Imaizumi T, Yagi K, Matsumoto Y, Nakashima T, Hirose A, Kashima N, Nosaka Y, Hamada T, Okawa K, Nishiya Y, Kubo K
Scientific reports 2019 May 23;9(1):7742

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Mutations in sphingosine-1-phosphate lyase cause nephrosis with ichthyosis and adrenal insufficiency.

Lovric S, Goncalves S, Gee HY, Oskouian B, Srinivas H, Choi WI, Shril S, Ashraf S, Tan W, Rao J, Airik M, Schapiro D, Braun DA, Sadowski CE, Widmeier E, Jobst-Schwan T, Schmidt JM, Girik V, Capitani G, Suh JH, Lachaussée N, Arrondel C, Patat J, Gribouval O, Furlano M, Boyer O, Schmitt A, Vuiblet V, Hashmi S, Wilcken R, Bernier FP, Innes AM, Parboosingh JS, Lamont RE, Midgley JP, Wright N, Majewski J, Zenker M, Schaefer F, Kuss N, Greil J, Giese T, Schwarz K, Catheline V, Schanze D, Franke I, Sznajer Y, Truant AS, Adams B, Désir J, Biemann R, Pei Y, Ars E, Lloberas N, Madrid A, Dharnidharka VR, Connolly AM, Willing MC, Cooper MA, Lifton RP, Simons M, Riezman H, Antignac C, Saba JD, Hildebrandt F
The Journal of clinical investigation 2017 Mar 1;127(3):912-928

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Expression of bioactive lysophospholipids and processing enzymes in the vitreous from patients with proliferative diabetic retinopathy.

Abu El-Asrar AM, Nawaz MI, Mohammad G, Siddiquei MM, Alam K, Mousa A, Opdenakker G
Lipids in health and disease 2014 Dec 11;13:187

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Increased expression of sphingosine kinase in the amnion during labor.

Erkhembaatar LO, Kotani T, Sumigama S, Tsuda H, Mano Y, Hua L, Hasegawa Y, Wang J, Sugiyama C, Nakahara T, Iwase A, Kikkawa F
Placenta 2013 Apr;34(4):353-9

Western blot

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Human SGPL1 by Western Blot. Western blot shows lysates of HEK293 human embryonic kidney cell line, HepG2 human hepatocellular carcinoma cell line, HT-29 human colon adenocarcinoma cell line, and HT-29 tumor tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human SGPL1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5535) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for SGPL1 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Human SGPL1 by Simple Western^TM. Simple Western lane view shows lysates of HEK293 human embryonic kidney cell line and HepG2 human hepatocellular carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for SGPL1 at approximately 60 kDa (as indicated) using 50 µg/mL of Goat Anti-Human SGPL1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5535) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.